| Nickel-induced changes in carbon metabolism in wheat shoots. | |
| | |
MedLine Citation:
|
PMID: 23374731 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
In this study, we analyzed the toxic effect of Ni during the development of wheat shoots. Typical developmental alterations in carbon metabolism-related parameters reflecting changes associated with the transition of the seedlings from heterotrophic to autotrophic metabolism were observed in the control shoots between the 1st and the 4th days. Adverse effects of 50 and 100μM Ni became evident starting from the 4th day of growth on the metal-containing media. We found that Ni-induced stimulation of phosphoenolpyruvate carboxylase (PEPC) activity coincided with decrease in the ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) level and with declines in net photosynthetic rate (P(N)) and stomatal conductance (g(s)). Application of Ni resulted in increased activities of several dehydrogenases: glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconate dehydrogenase (6PGDH), isocitrate dehydrogenase (NADP-ICDH) and malate dehydrogenase (NADH-MDH). In contrast, the activities of malic enzymes (NADP-ME and NAD-ME) decreased due to Ni stress. Treatment with Ni led to accumulation of glucose and declined concentration of sucrose as well as considerable increases in concentrations of malic and citric acids. Our results indicate that Ni stress redirects the carbon metabolism of developing wheat shoots to provide carbon skeletons for synthesis of amino acids and organic acids as well as to supply reducing power to sustain normal metabolic processes and to support defense mechanisms against oxidative stress. |
| | |
Authors:
|
Ewa Gajewska; Ewa Niewiadomska; Krzysztof Tokarz; Mirosława Słaba; Maria Skłodowska |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of plant physiology Volume: 170 ISSN: 1618-1328 ISO Abbreviation: J. Plant Physiol. Publication Date: 2013 Mar |
Date Detail:
|
Created Date: 2013-02-04 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9882059 Medline TA: J Plant Physiol Country: Germany |
Other Details:
|
Languages: eng Pagination: 369-77 Citation Subset: IM |
Copyright Information:
|
Copyright © 2012 Elsevier GmbH. All rights reserved. |
Affiliation:
|
Department of Plant Physiology and Biochemistry, Faculty of Biology and Environmental Protection, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland. Electronic address: ewagaj@biol.uni.lodz.pl. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Quantitation of cell-free DNA and RNA in plasma during tumor progression in rats.
Next Document: Evaluation of fracture healing and subimplant bone response following fixation with a locking minipl...