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Nickel-induced changes in carbon metabolism in wheat shoots.
MedLine Citation:
PMID:  23374731     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
In this study, we analyzed the toxic effect of Ni during the development of wheat shoots. Typical developmental alterations in carbon metabolism-related parameters reflecting changes associated with the transition of the seedlings from heterotrophic to autotrophic metabolism were observed in the control shoots between the 1st and the 4th days. Adverse effects of 50 and 100μM Ni became evident starting from the 4th day of growth on the metal-containing media. We found that Ni-induced stimulation of phosphoenolpyruvate carboxylase (PEPC) activity coincided with decrease in the ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) level and with declines in net photosynthetic rate (P(N)) and stomatal conductance (g(s)). Application of Ni resulted in increased activities of several dehydrogenases: glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconate dehydrogenase (6PGDH), isocitrate dehydrogenase (NADP-ICDH) and malate dehydrogenase (NADH-MDH). In contrast, the activities of malic enzymes (NADP-ME and NAD-ME) decreased due to Ni stress. Treatment with Ni led to accumulation of glucose and declined concentration of sucrose as well as considerable increases in concentrations of malic and citric acids. Our results indicate that Ni stress redirects the carbon metabolism of developing wheat shoots to provide carbon skeletons for synthesis of amino acids and organic acids as well as to supply reducing power to sustain normal metabolic processes and to support defense mechanisms against oxidative stress.
Authors:
Ewa Gajewska; Ewa Niewiadomska; Krzysztof Tokarz; Mirosława Słaba; Maria Skłodowska
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of plant physiology     Volume:  170     ISSN:  1618-1328     ISO Abbreviation:  J. Plant Physiol.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-04     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882059     Medline TA:  J Plant Physiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  369-77     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier GmbH. All rights reserved.
Affiliation:
Department of Plant Physiology and Biochemistry, Faculty of Biology and Environmental Protection, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland. Electronic address: ewagaj@biol.uni.lodz.pl.
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