Document Detail


Next-generation sequencing of multiple individuals per barcoded library by deconvolution of sequenced amplicons using endonuclease fragment analysis.
MedLine Citation:
PMID:  25109295     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The simultaneous sequencing of samples from multiple individuals increases the efficiency of next-generation sequencing (NGS) while also reducing costs. Here we describe a novel and simple approach for sequencing DNA from multiple individuals per barcode. Our strategy relies on the endonuclease digestion of PCR amplicons prior to library preparation, creating a specific fragment pattern for each individual that can be resolved after sequencing. By using both barcodes and restriction fragment patterns, we demonstrate the ability to sequence the human melanocortin 1 receptor (MC1R) genes from 72 individuals using only 24 barcoded libraries.
Authors:
Jeppe D Andersen; Vania Pereira; Carlotta Pietroni; Martin Mikkelsen; Peter Johansen; Claus Børsting; Niels Morling
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Publication Detail:
Type:  Journal Article     Date:  2014-08-01
Journal Detail:
Title:  BioTechniques     Volume:  57     ISSN:  1940-9818     ISO Abbreviation:  BioTechniques     Publication Date:  2014  
Date Detail:
Created Date:  2014-08-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8306785     Medline TA:  Biotechniques     Country:  England    
Other Details:
Languages:  eng     Pagination:  91-4     Citation Subset:  IM    
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