Document Detail


Next generation planar waveguide detection of microcystins in freshwater and cyanobacterial extracts, utilising a novel lysis method for portable sample preparation and analysis.
MedLine Citation:
PMID:  23498128     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The study details the development of a fully validated, rapid and portable sensor based method for the on-site analysis of microcystins in freshwater samples. The process employs a novel lysis method for the mechanical lysis of cyanobacterial cells, with glass beads and a handheld frother in only 10min. The assay utilises an innovative planar waveguide device that, via an evanescent wave excites fluorescent probes, for amplification of signal in a competitive immunoassay, using an anti-microcystin monoclonal with cross-reactivity against the most common, and toxic variants. Validation of the assay showed the limit of detection (LOD) to be 0.78ngmL(-1) and the CCβ to be 1ngmL(-1). Robustness of the assay was demonstrated by intra- and inter-assay testing. Intra-assay analysis had % C.V.s between 8 and 26% and recoveries between 73 and 101%, with inter-assay analysis demonstrating % C.V.s between 5 and 14% and recoveries between 78 and 91%. Comparison with LC-MS/MS showed a high correlation (R(2)=0.9954) between the calculated concentrations of 5 different Microcystis aeruginosa cultures for total microcystin content. Total microcystin content was ascertained by the individual measurement of free and cell-bound microcystins. Free microcystins can be measured to 1ngmL(-1), and with a 10-fold concentration step in the intracellular microcystin protocol (which brings the sample within the range of the calibration curve), intracellular pools may be determined to 0.1ngmL(-1). This allows the determination of microcystins at and below the World Health Organisation (WHO) guideline value of 1μgL(-1). This sensor represents a major advancement in portable analysis capabilities and has the potential for numerous other applications.
Authors:
Shauna Devlin; Julie P Meneely; Brett Greer; Charles Greef; Michael J Lochhead; Christopher T Elliott
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Publication Detail:
Type:  Journal Article     Date:  2013-01-24
Journal Detail:
Title:  Analytica chimica acta     Volume:  769     ISSN:  1873-4324     ISO Abbreviation:  Anal. Chim. Acta     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-03-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370534     Medline TA:  Anal Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  108-13     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Elsevier B.V. All rights reserved.
Affiliation:
Institute for Global Food Security (IGFS), School of Biological Sciences, Queen's University, Malone Road, 18-30 Belfast, BT9 5BN, Northern Ireland, United Kingdom. Electronic address: s.devlin@qub.ac.uk.
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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