Document Detail


Newly proposed fibrosis staging criterion for assessing carbon tetrachloride- and albumin complex-induced liver fibrosis in rodents.
MedLine Citation:
PMID:  18801073     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The fibrosis staging criterion (Knodell's) for viral hepatitis in human should not be applied to experimental liver fibrosis in rodents because of differences of species and etiology. Liver fibrosis was induced by carbon tetrachloride (CCl(4)) or albumin antigen-antibody complex in Balb/C mice or Wistar rats. The degree of fibrosis was quantified by staging scores or hydroxyproline measurement or image analysis. Inter- and intra-observer variations of the criterion were also evaluated. Liver fibrosis was divided into seven stages: stage 0, no fibrosis; stage 1, short fibrous tissue in central venule (C); stage 2, fibrous C-C septa appearance; stage 3, C-C fibrous septa developed incompletely; stage 4, C-C septa completely connected (pseudo-lobule); stage 5, C-P (Portal Tract) bridging fibrosis, nodular appearance < or =50%.; and stage 6, nodular appearance >50%. There was significant correlation between the staging scores and hydroxyproline concentration (r = 0.708 in mice, r = 0.841 in rats) and, between staging scores and fibrosis area (r = 0.919 in rats, P < 0.001). The interobserver and intraobserver agreement was consistent (kappa = 0.738 or 0.726, P < 0.001). This staging of hepatic fibrosis in rodents is scientifically rational and repeatable, and is therefore suggested as the criterion for the assessment of experimental hepatic fibrosis in rodent studies.
Authors:
Xin-Yan Zhao; Bao-En Wang; Xin-Min Li; Tai-Ling Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Pathology international     Volume:  58     ISSN:  1440-1827     ISO Abbreviation:  Pathol. Int.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-09-19     Completed Date:  2008-11-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9431380     Medline TA:  Pathol Int     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  580-8     Citation Subset:  IM    
Affiliation:
Liver Research Center, Beijing Friendship Hospital, Capital Medical University.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigen-Antibody Complex / immunology
Carbon Tetrachloride
Humans
Hydroxyproline / metabolism
Image Processing, Computer-Assisted
Liver Cirrhosis, Experimental / chemically induced,  classification,  metabolism,  pathology*
Male
Mice
Mice, Inbred BALB C
Observer Variation
Rats
Rats, Wistar
Reproducibility of Results
Serum Albumin
Chemical
Reg. No./Substance:
0/Antigen-Antibody Complex; 0/Serum Albumin; 51-35-4/Hydroxyproline; 56-23-5/Carbon Tetrachloride

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