| Newly identified CpG ODNs, M5-30 and M6-395, stimulate mouse immune cells to secrete TNF-alpha and enhance Th1-mediated immunity. | |
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MedLine Citation:
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PMID: 20799094 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bacterial CpG motifs are known to induce both innate and adaptive immunity in infected hosts via toll-like receptor 9 (TLR9). Because small oligonucleotides (ODNs) mimicking bacterial CpG motifs are easily synthesized, they have found use as immunomodulatory agents in a number of disease models. We have developed a novel bioinformatics approach to identify effective CpG ODN sequences and evaluate their function as TLR9 ligands in a murine system. Among the CpG ODNs we identified, M5-30 and M6-395 showed significant ability to stimulate TNF-alpha and IFN-gamma production in a mouse macrophage cell line and mouse splenocytes, respectively. We also found that these CpG ODNs activated cells through the canonical NF-kappa B signaling pathway. Moreover, both CpG ODNs were able to induce Th1-mediated immunity in Mycobacterium tuberculosis (Mtb)-infected mice. Our results demonstrate that M5-30 and M6-395 function as TLR9-specific ligands, making them useful in the study of TLR9 functionality and signaling in mice. |
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Authors:
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Sun-Shim Choi; Eunkyung Chung; Yu-Jin Jung |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-20 |
Journal Detail:
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Title: Journal of microbiology (Seoul, Korea) Volume: 48 ISSN: 1976-3794 ISO Abbreviation: J. Microbiol. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-08-27 Completed Date: 2010-12-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9703165 Medline TA: J Microbiol Country: Korea (South) |
Other Details:
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Languages: eng Pagination: 512-7 Citation Subset: IM |
Affiliation:
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Department of Molecular and Medical Biotechnology, Kangwon National University, Chuncheon, Republic of Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Cell Line Cells, Cultured Humans Immunity, Cellular* Interferon-gamma / immunology Macrophages / immunology Mice / genetics, immunology* Mice, Inbred C57BL Molecular Sequence Data Mycobacterium tuberculosis / physiology Oligodeoxyribonucleotides / chemistry, immunology* Spleen / cytology, immunology Th1 Cells / immunology* Toll-Like Receptor 9 / genetics, immunology Tuberculosis / immunology, microbiology Tumor Necrosis Factor-alpha / immunology* |
| Chemical | |
Reg. No./Substance:
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0/CPG-oligonucleotide; 0/Oligodeoxyribonucleotides; 0/Toll-Like Receptor 9; 0/Tumor Necrosis Factor-alpha; 82115-62-6/Interferon-gamma |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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