Document Detail

Newly delivered transferrin iron and oxidative cell injury.
MedLine Citation:
PMID:  9042969     Owner:  NLM     Status:  MEDLINE    
Cell iron status was assessed in terms of its capacity to mediate cell injury by pro-oxidants. Cultured K562 cells, which maintain a stable cytosolic labile iron pool (LIP) of < 0.5 microM, underwent distinct changes after short exposures to transferrin (Tf) followed by t-butyl hydroperoxide (TBHP): (a) rise in LIP, detectable fluorimetrically; (b) increased lipid peroxidation and (c) eventual cell death. All of these effects were inhibited by weak bases or iron chelators. Similarly, hydrogen peroxide caused rises in both LIP and oxidant species detectable with 2',7'-dichlorofluorescin diacetate, which were enhanced by preincubation with Tf. The Tf-delivered iron disappeared from LIP and the TBHP-reactive pool with a t1/2 < 30 min. The results indicate that the catalytic potential of iron is highest while in transit between endosomes and cytosolic ligands.
W Breuer; E Greenberg; Z I Cabantchik
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  FEBS letters     Volume:  403     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1997 Feb 
Date Detail:
Created Date:  1997-03-20     Completed Date:  1997-03-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  213-9     Citation Subset:  IM    
Department of Biological Chemistry, Institute of Life Sciences, Hebrew University of Jerusalem, Israel. BILLB@VMS.HUJI.AC.IL
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MeSH Terms
Cell Survival
Fluoresceins / chemistry
Hydrogen Peroxide / pharmacology
Iron / metabolism*
Iron Chelating Agents / pharmacology
Malondialdehyde / metabolism
Oxidative Stress*
Peroxides / pharmacology
Transferrin / metabolism*
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:
0/Fluoresceins; 0/Iron Chelating Agents; 0/Peroxides; 11096-37-0/Transferrin; 2044-85-1/diacetyldichlorofluorescein; 542-78-9/Malondialdehyde; 7439-89-6/Iron; 75-91-2/tert-Butylhydroperoxide; 7722-84-1/Hydrogen Peroxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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