| Newly delivered transferrin iron and oxidative cell injury. | |
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MedLine Citation:
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PMID: 9042969 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell iron status was assessed in terms of its capacity to mediate cell injury by pro-oxidants. Cultured K562 cells, which maintain a stable cytosolic labile iron pool (LIP) of < 0.5 microM, underwent distinct changes after short exposures to transferrin (Tf) followed by t-butyl hydroperoxide (TBHP): (a) rise in LIP, detectable fluorimetrically; (b) increased lipid peroxidation and (c) eventual cell death. All of these effects were inhibited by weak bases or iron chelators. Similarly, hydrogen peroxide caused rises in both LIP and oxidant species detectable with 2',7'-dichlorofluorescin diacetate, which were enhanced by preincubation with Tf. The Tf-delivered iron disappeared from LIP and the TBHP-reactive pool with a t1/2 < 30 min. The results indicate that the catalytic potential of iron is highest while in transit between endosomes and cytosolic ligands. |
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Authors:
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W Breuer; E Greenberg; Z I Cabantchik |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: FEBS letters Volume: 403 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1997 Feb |
Date Detail:
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Created Date: 1997-03-20 Completed Date: 1997-03-20 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 213-9 Citation Subset: IM |
Affiliation:
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Department of Biological Chemistry, Institute of Life Sciences, Hebrew University of Jerusalem, Israel. BILLB@VMS.HUJI.AC.IL |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Survival Fluoresceins / chemistry Humans Hydrogen Peroxide / pharmacology Iron / metabolism* Iron Chelating Agents / pharmacology Malondialdehyde / metabolism Oxidative Stress* Peroxides / pharmacology Transferrin / metabolism* Tumor Cells, Cultured tert-Butylhydroperoxide |
| Grant Support | |
ID/Acronym/Agency:
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AI20342/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fluoresceins; 0/Iron Chelating Agents; 0/Peroxides; 11096-37-0/Transferrin; 2044-85-1/diacetyldichlorofluorescein; 542-78-9/Malondialdehyde; 7439-89-6/Iron; 75-91-2/tert-Butylhydroperoxide; 7722-84-1/Hydrogen Peroxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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