Document Detail


Newcastle disease virus-induced apoptosis in PC12 pheochromocytoma cells.
MedLine Citation:
PMID:  12845392     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The avian paramyxovirus Newcastle disease virus (NDV) causes severe infections in birds. It is essentially nonpathogenic in rodents and human beings but was found to have an oncolytic potential against certain types of human malignancies. An attenuated NDV vaccine (designated MTH-68/H) was found to cause regression of various human tumors, but the mechanism of its oncolytic action and its selectivity toward malignant cells remain poorly understood. NDV was reported to cause apoptotic death in several avian cultured cell types. Programmed cell death may thus be the basis for the oncolytic effect of NDV vaccines. To test this possibility, we chose the PC12 rat pheochromocytoma cell line, a widely used model system for apoptosis. The MTH-68/H vaccine was found to cause apoptotic death of PC12 cells in a dose-dependent manner. A brief exposure of cells to the virus was found to trigger the apoptotic response. Cell death induced by the vaccine was not accompanied by significant alterations in the major mitogen-activated protein kinase pathways of these cells. Apoptotic DNA fragmentation was not affected by stimulating growth factor pathways or signaling mechanisms mediated by protein kinase C or the second messenger, calcium. In contrast, stimulation of protein kinase A by cyclic adenosine monophosphate analogs gave partial protection against the virus. PC12 cells thus provide a useful model system to study the effects of NDV on cell survival at the molecular level.
Authors:
József Szeberényi; Zsolt Fábián; Beáta Töröcsik; Katalin Kiss; Laszlo K Csatary
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  American journal of therapeutics     Volume:  10     ISSN:  1075-2765     ISO Abbreviation:  Am J Ther     Publication Date:    2003 Jul-Aug
Date Detail:
Created Date:  2003-07-07     Completed Date:  2003-11-05     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9441347     Medline TA:  Am J Ther     Country:  United States    
Other Details:
Languages:  eng     Pagination:  282-8     Citation Subset:  IM    
Affiliation:
Department of Medical Biology, School of Medicine, University of Pécs, Pécs, Hungary. jozsef.szeberenyi@aok.pte.hu
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects*
Clinical Trials as Topic
DNA Fragmentation / drug effects
Humans
Mitogen-Activated Protein Kinase Kinases / drug effects
PC12 Cells / drug effects*
Rats
Signal Transduction / drug effects*
Viral Vaccines / pharmacology*,  therapeutic use
Chemical
Reg. No./Substance:
0/Newcastle disease virus vaccine MTH-68-H; 0/Viral Vaccines; EC 2.7.12.2/Mitogen-Activated Protein Kinase Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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