| Newcastle disease virus activates macrophages for anti-tumor activity. | |
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MedLine Citation:
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PMID: 10639582 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Newcastle Disease Virus (NDV), an agent with interesting immune stimulatory and anti-tumor activity, was investigated for its capacity to activate anti-tumor activity in murine macrophages in vitro and in vivo. Direct macrophage activation was seen under a variety of experimental conditions using two different strains of NDV, different sources of macrophages (spleen and peritoneum) and different strains of mice (DBA/2, C57BL/6, 615). Various macrophage enzymes (ADA, iNOS, lysozyme, acid phosphatase) became upregulated and anti-tumor effector molecules such as nitric oxide (NO) and TNF-alpha were found in the supernatant. NDV activated macrophages performed anti-tumor activity in vitro such as anti-tumor cytostasis and anti-tumor cytotoxicity. The cytotoxic anti-tumor activity was broad and active against all tumor lines tested including mammary carcinoma, lung carcinoma, mastocytoma and immune escape variants (lymphoma). Macrophage activation via BCG/LPS also caused a broad range anti-tumor cytotoxic activity while activation via mixed lymphocyte culture conditioned medium had restricted anti-tumor activity. Anti-tumor activity of NDV activated macrophages could be transfered in vivo. Transfer of macrophages which had not been appropriately activated exerted either no effect or a tumor growth augmenting effect. Repeated intravenous transfer of NDV activated macrophages exerted a significant suppressive effect on pulmonary metastases in a mammary carcinoma tumor model as well as in a lung carcinoma model. Taken together these results demonstrate that NDV can strongly activate macrophages to perform anti-tumor activities in vitro and in vivo. |
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Authors:
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V Schirrmacher; L Bai; V Umansky; L Yu; Y Xing; Z Qian |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: International journal of oncology Volume: 16 ISSN: 1019-6439 ISO Abbreviation: Int. J. Oncol. Publication Date: 2000 Feb |
Date Detail:
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Created Date: 2000-03-07 Completed Date: 2000-03-07 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 9306042 Medline TA: Int J Oncol Country: GREECE |
Other Details:
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Languages: eng Pagination: 363-73 Citation Subset: IM |
Affiliation:
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German Cancer Research Center (DKFZ), Division of Cellular Immunology, D-69120 Heidelberg, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Female Lung Neoplasms / therapy* Macrophage Activation* Macrophages / immunology*, virology Mammary Neoplasms, Animal / therapy* Mice Mice, Inbred C57BL Mice, Inbred DBA Newcastle disease virus / immunology* Nitric Oxide / metabolism Tumor Necrosis Factor-alpha / drug effects, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Tumor Necrosis Factor-alpha; 10102-43-9/Nitric Oxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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