Document Detail


New three-stage in vitro model for infant colonic fermentation with immobilized fecal microbiota.
MedLine Citation:
PMID:  16867149     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The development and validation of a new three-stage culture system with immobilized fecal microbiota to simulate infant colonic ecosystem is described. Two continuous cultures with different fecal inocula were used to assess the validity and stability of the intestinal model. The total anaerobe populations measured in beads and effluent fermentations reached high concentrations similar to infant feces. Fluorescence in situ hybridization analyses and denaturing gradient gel electrophoresis profiles of effluent samples from the three reactors revealed complex patterns similar to that observed in the inoculum, indicating that fecal bacterial diversity was well-preserved and that dominant bacterial populations showed good stability among reactors. For both experiments, the bacterial populations and fermentation product concentrations were in the range of published data for infant feces. These results demonstrate that this new three-stage continuous culture with immobilized cells provides a useful tool for studying the infant colon ecosystem.
Authors:
Cécile Cinquin; Gwenaëlle Le Blay; Ismaïl Fliss; Christophe Lacroix
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS microbiology ecology     Volume:  57     ISSN:  0168-6496     ISO Abbreviation:  FEMS Microbiol. Ecol.     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-07-26     Completed Date:  2006-09-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8901229     Medline TA:  FEMS Microbiol Ecol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  324-36     Citation Subset:  IM    
Affiliation:
Dairy Research Centre STELA, Pavillon Paul Comtois, Université Laval, Québec, QC, Canada.
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MeSH Terms
Descriptor/Qualifier:
Bacteria / metabolism*
Biodiversity
Cells, Immobilized*
Colon / microbiology*
Colony Count, Microbial
DNA Fingerprinting
DNA, Bacterial / analysis,  genetics
Ecosystem
Electrophoresis, Gel, Two-Dimensional
Feces / microbiology
Fermentation
Humans
In Situ Hybridization
Infant
Microscopy, Confocal
Microspheres
Models, Biological*
Nucleic Acid Denaturation
Chemical
Reg. No./Substance:
0/DNA, Bacterial
Comments/Corrections
Erratum In:
FEMS Microbiol Ecol. 2006 Aug;57(2):340-2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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