Document Detail

New technologies for dissecting the arteriolar myogenic response.
MedLine Citation:
PMID:  17573129     Owner:  NLM     Status:  MEDLINE    
The arteriolar myogenic response is crucial for the setting of vascular resistance and for providing a level of tone upon which vasodilators can act. Despite its physiological importance, questions remain regarding the underlying signaling mechanisms of the arteriolar myogenic response. Does an increase in pressure within an arteriole exert its effects via the extracellular matrix, an action on cell membranes and/or deformation of cytoskeletal structures? Recent advances in methodology, particularly involving sophisticated imaging approaches, are enabling the study of forces at single-cell and even subcellular levels. Atomic force microscopy (AFM) not only enables detection of cell morphology and stiffness but also allows discrete forces to be applied to single smooth muscle cells and subsequent responses to be observed. Importantly, the repertoire of approaches involving AFM can be expanded by using it in combination with other imaging approaches - including fluorescence imaging for cellular signals such as Ca(2+), and total internal reflectance fluorescence, fluorescence resonance energy transfer and confocal microscopy for probing cellular contact function. Combinations of these advanced imaging and nanomechanical approaches will be instructive to studies of intact vessels and the circulatory system in general.
Michael A Hill; Zhe Sun; Luis Martinez-Lemus; Gerald A Meininger
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Publication Detail:
Type:  Journal Article; Review     Date:  2007-06-18
Journal Detail:
Title:  Trends in pharmacological sciences     Volume:  28     ISSN:  0165-6147     ISO Abbreviation:  Trends Pharmacol. Sci.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-07-09     Completed Date:  2007-11-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7906158     Medline TA:  Trends Pharmacol Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  308-15     Citation Subset:  IM    
Dalton Cardiovascular Research Center and Department of Medical Pharmacology and Physiology, University of Missouri, Columbia, MO 65211, USA.
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MeSH Terms
Arterioles / physiology*
Extracellular Matrix / physiology
Fluorescence Resonance Energy Transfer
Microscopy, Atomic Force
Microscopy, Confocal
Microscopy, Fluorescence
Myocytes, Smooth Muscle / physiology*

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