Document Detail


New techniques for isolation of single prokaryotic cells.
MedLine Citation:
PMID:  11077150     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Since the 1960s, several new attempts have been made to improve the management of single prokaryotic cells using micromanipulator techniques. In order to facilitate the isolation of pure cultures we have recently developed an improved micromanipulation method for routine work. With the aid of this method single prokaryotic cells can be picked out of a mixed community under direct visual control. The isolated aerobic or anaerobic cells can be grown in pure culture or can be subjected to single cell PCR. Other powerful and completely new approaches are the applications of laser micromanipulation systems, such as optical tweezers or laser microdissection techniques. Of the latter two methods only optical tweezers have been successfully applied to cloning prokaryotic cells.
Authors:
J Fröhlich; H König
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  FEMS microbiology reviews     Volume:  24     ISSN:  0168-6445     ISO Abbreviation:  FEMS Microbiol. Rev.     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2000-12-08     Completed Date:  2001-02-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8902526     Medline TA:  FEMS Microbiol Rev     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  567-72     Citation Subset:  IM    
Affiliation:
Institut für Mikrobiologie und Weinforschung, Johannes Gutenberg-Universität, Becherweg 15, 55128, Mainz, Germany.
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MeSH Terms
Descriptor/Qualifier:
Bacteria / cytology,  growth & development,  isolation & purification*
Bacteriological Techniques*
Ecosystem
Lasers
Micromanipulation / methods*

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