| New technique for the cytologic identification of presumed Acanthamoeba from corneal epithelial scrapings. | |
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MedLine Citation:
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PMID: 10030566 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To describe a cytologic technique for the rapid identification of presumed Acanthamoeba organisms from corneal epithelial scrapings. METHODS: After administering topical anesthesia, we removed the affected corneal epithelium with a scalpel blade. The tip of the blade, containing the scrapings, was washed off into a cuvette with a solution of an alcohol-based fixative for cytology specimens. The blade was immersed in the cuvette and agitated to ensure that the sample was collected. The specimen was fixed for at least 10 minutes and processed by cytospin centrifugation. RESULT: Seventy-five patient samples have been studied with this technique, with excellent preservation of the organism. CONCLUSIONS: The organism preservation with this technique is superior to that of conventional smears and permits confirmatory organism identification by immunohistochemistry. |
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Authors:
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L M Gardner; W D Mathers; R Folberg |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: American journal of ophthalmology Volume: 127 ISSN: 0002-9394 ISO Abbreviation: Am. J. Ophthalmol. Publication Date: 1999 Feb |
Date Detail:
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Created Date: 1999-02-26 Completed Date: 1999-02-26 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370500 Medline TA: Am J Ophthalmol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 207-9 Citation Subset: AIM; IM |
Affiliation:
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Department of Ophthalmology & Visual Sciences, University of Iowa, Iowa City 51142-1182, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acanthamoeba
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cytology*,
immunology Acanthamoeba Keratitis / diagnosis* Anesthesia, Local Animals Antibodies, Protozoan / diagnostic use Antigens, Protozoan / analysis Diagnostic Techniques, Ophthalmological* Epithelium, Corneal / parasitology* Humans Immunoenzyme Techniques Tissue Fixation |
| Grant Support | |
ID/Acronym/Agency:
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EY11667/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Protozoan; 0/Antigens, Protozoan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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