Document Detail


New target tissue for food-borne virus detection in oysters.
MedLine Citation:
PMID:  19146529     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To evaluate the different tissues of naturally contaminated oyster for food-borne virus detection.
METHODS AND RESULTS: The different tissues of 136 field oyster samples were analysed for norovirus (NV), hepatitis A virus (HAV) and rotavirus (RV) by reverse transcription (RT)-PCR and were confirmed by sequencing. These viruses were detected in 20 samples (14.71%), showing positivity for NV (1.47%), HAV (5.15%) and RV (8.82%). Furthermore, among different tissues, the highest positive rate of the food-borne viruses was found in the gills (14.71%), followed by the stomach (13.97%) and the digestive diverticula (13.24%).
CONCLUSIONS: The food-borne viruses were detected in the gills, stomach, digestive diverticula and the cilia of the mantle. In addition, the results showed that the gills are one of the appropriate tissues for viral detection in oysters by nucleic acid assay.
SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first paper to report on the presence of food-borne viruses in the gills and the cilia of the mantle of naturally contaminated oysters. The research team hopes that the results of the study will be of help in sampling the appropriate tissues for the detection of food-borne viruses in commercial oysters.
Authors:
D Wang; Q Wu; L Yao; M Wei; X Kou; J Zhang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Letters in applied microbiology     Volume:  47     ISSN:  1472-765X     ISO Abbreviation:  Lett. Appl. Microbiol.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2009-01-16     Completed Date:  2009-02-16     Revised Date:  2014-07-30    
Medline Journal Info:
Nlm Unique ID:  8510094     Medline TA:  Lett Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  405-9     Citation Subset:  IM    
Data Bank Information
Bank Name/Acc. No.:
GENBANK/EU554058;  EU554059;  EU554060;  EU554061;  EU554062;  EU554063;  EU554064;  EU554065;  EU554066;  EU554067;  EU704687;  EU704688;  EU704689;  EU704690;  EU704691;  EU704692;  EU704693;  EU704694;  EU704695
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Gills / virology
Hepatitis A virus / isolation & purification*
Molecular Sequence Data
Norovirus / isolation & purification*
Ostreidae / virology*
RNA, Viral / genetics
Reverse Transcriptase Polymerase Chain Reaction
Rotavirus / isolation & purification*
Sequence Analysis, DNA
Stomach / virology
Tissue Culture Techniques / methods*
Virus Cultivation / methods
Virus Diseases / veterinary*
Chemical
Reg. No./Substance:
0/RNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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