| New substituted aryldiazomethyl labels for high density DNA chip-based nucleic acid testing. | |
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MedLine Citation:
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PMID: 18776349 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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DNA and RNA labeling and detection are key steps in nucleic acid testing, particularly for molecular diagnostics applications. Here, we report a new class of aryldiazomethyl labels that include in their structure a biotin moiety as a detectable unit and a nitro substituted phenyl diazomethyl as a reactive group. The greatest reactivity towards phosphates of nucleic acids, the water solubility and the stability of these new molecules were demonstrated. These very important properties, which are the main requirements for nucleic acid labeling in aqueous conditions using automated protocols within integrated diagnostic devices, make them the perfect labeling tools for hybridization-based analysis, especially for high-density DNA chips. |
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Authors:
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Alain Laurent; Mitsuharu Kotera; Arnaud Burr; Frederick Lasnet; Thibault Martin; Ali Laayoun |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Nucleic acids symposium series (2004) Volume: - ISSN: 1746-8272 ISO Abbreviation: Nucleic Acids Symp Ser (Oxf) Publication Date: 2008 |
Date Detail:
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Created Date: 2008-09-08 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101259965 Medline TA: Nucleic Acids Symp Ser (Oxf) Country: England |
Other Details:
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Languages: eng Pagination: 253-4 Citation Subset: IM |
Affiliation:
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bioMerieux, R&D Molecular Technologies, Christophe Merieux Center, 5 rue des Berges, 38024 Cedex 01 Grenoble, France. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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