| New strategy for the determination of gliadins in maize- or rice-based foods matrix-assisted laser desorption/ionization time-of-flight mass spectrometry: fractionation of gliadins from maize or rice prolamins by acidic treatment. | |
| | |
MedLine Citation:
|
PMID: 12938107 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A procedure for determining small quantities of gliadins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) in gluten-free foods containing relatively large amounts of prolamin proteins from maize or rice is described. We report for the first time that gliadins, the ethanol-soluble wheat prolamin fraction, can be quantitatively solubilized in 1.0 M acetic acid, while the corresponding ethanol-soluble maize or rice prolamin fraction remains insoluble in acetic acid. We describe a methodology for the detection of gliadins in maize and rice foods based on a two-step procedure of extraction (60% aqueous ethanol followed by 1 M acetic acid). Subsequent MALDI-TOFMS analysis of the resulting acidic extract from these gluten-free foods clearly confirms the presence of a typical mass pattern corresponding to gliadin components, ranging from 30 to 45 kDa. Depending on the percentages of maize or rice flours employed in the elaboration of these foods, the combined procedure enables levels of gliadins from 100 to 400 ppm to be detected. The efficiency of this combined procedure corroborates enzyme-linked immunosorbent assay data for a large number of maize/rice gluten-free foods by means of direct visualization of the characteristic gliadin mass pattern in maize or rice foods. |
| | |
Authors:
|
Alberto Hernando; Israel Valdes; Enrique Méndez |
Related Documents
:
|
22052727 - Effects of specific egg yolk antibody (igy) on the quality and shelf life of refrigerat... 18686967 - Potential food additives from carex distachya roots: identification and in vitro antiox... 4019987 - Salicylates in foods. 9311147 - Matrix-assisted laser desorption/ionization time-of-flight mass spectrometric micro-ana... 22691997 - Effects of stenochlaena palustris leaf extract on growth and morphogenesis of food born... 18636367 - An investigation into the bioaccessibility of potassium in unprocessed fruits and veget... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of mass spectrometry : JMS Volume: 38 ISSN: 1076-5174 ISO Abbreviation: J Mass Spectrom Publication Date: 2003 Aug |
Date Detail:
|
Created Date: 2003-08-25 Completed Date: 2003-09-24 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9504818 Medline TA: J Mass Spectrom Country: England |
Other Details:
|
Languages: eng Pagination: 862-71 Citation Subset: IM |
Copyright Information:
|
Copyright 2003 John Wiley & Sons, Ltd. |
Affiliation:
|
Unidad de Gluten, Centro Nacional de Biotecnología, Canto Blanco, E-28049 Madrid, Spain. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Food Analysis
/
methods* Gliadin / analysis* Oryza sativa / chemistry* Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods* Zea mays / chemistry* |
| Chemical | |
Reg. No./Substance:
|
9007-90-3/Gliadin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Gas-phase hydrogen/deuterium exchange of adenine nucleotides.
Next Document: Stable isotope labeling for matrix-assisted laser desorption/ionization mass spectrometry and post-s...