Document Detail

New strategies to establish human monoclonal antibodies.
MedLine Citation:
PMID:  1306317     Owner:  NLM     Status:  MEDLINE    
In order to establish human monoclonal antibodies to any sort of antigens efficiently, we have made following two approaches. Our first approach is to improve cell fusion frequency. By improving our previous method for production of human hybridomas, we obtained higher frequency (1/700 vs. 1/5500) compared with our previous method by adding irradiated myeloma cells to culture of fusion cells and modifying the selective medium. Our second approach is to use a SCID-hu mouse for immunization. Since the injection of human PBL can result in the stable long-term reconstitution of a human immune system in SCID mouse, we tried to immune SCID-hu mouse with KLH. In the serum of immunized SCID-hu mouse, we obtained human IgG antibodies to KLH. Additionally, we succeeded in establishing human B lymphoblastoid cell lines which produced antibodies specific to KLH. These methods will open new prospects for the detection and therapy of cancer.
T Kudo; H Saeki; S Saijyo; N Sato; M Numasaki; T Tachibana
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Tohoku journal of experimental medicine     Volume:  168     ISSN:  0040-8727     ISO Abbreviation:  Tohoku J. Exp. Med.     Publication Date:  1992 Oct 
Date Detail:
Created Date:  1993-08-05     Completed Date:  1993-08-05     Revised Date:  2013-11-14    
Medline Journal Info:
Nlm Unique ID:  0417355     Medline TA:  Tohoku J Exp Med     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  323-7     Citation Subset:  IM    
Department of Surgery, Tohoku University, Sendai, Japan.
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MeSH Terms
Antibodies, Monoclonal / biosynthesis*
Antibody Specificity
Antigens / immunology
B-Lymphocytes / immunology
Cell Fusion
Cell Line
Culture Media
Hemocyanin / immunology
Hybridomas / immunology*
Immunoglobulin G / blood
Mice, SCID
Multiple Myeloma / immunology
Ouabain / analysis
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens; 0/Culture Media; 0/Immunoglobulin G; 630-60-4/Ouabain; 9013-72-3/Hemocyanin; FV4Y0JO2CX/keyhole-limpet hemocyanin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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