| New strategies for nucleic acid delivery to conquer cellular and nuclear membranes. | |
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MedLine Citation:
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PMID: 18655814 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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After administration to the body, nucleic acid containing nanoparticles (NANs) need to cross several extra- and intracellular barriers to reach the cytoplasm or nucleus of the target cells. In the last decade several groups tried to overcome these barriers by arming non-viral delivery systems with targeting moieties, polyethylene glycol chains, fusogenic peptides and so forth. However, the drawback of this upgrading strategy is that each of the encountered barriers requires a new functionality, leading to very complex multi-component NANs. Moreover, there are currently no components available that can efficiently transport genes or NANs inside the nucleus of non-dividing cells. In this article a new, ultrasound based delivery system that possesses the capacity to simultaneously overcome several key barriers in non-viral nucleic acid delivery is presented. Additionally, a small amphiphilic compound that induces nuclear uptake of plasmid DNA and enhances non-viral gene transfer is presented. |
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Authors:
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Ine Lentacker; Roosmarijn E Vandenbroucke; Bart Lucas; Joseph Demeester; Stefaan C De Smedt; Niek N Sanders |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-07-04 |
Journal Detail:
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Title: Journal of controlled release : official journal of the Controlled Release Society Volume: 132 ISSN: 1873-4995 ISO Abbreviation: J Control Release Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2009-06-12 Completed Date: 2009-07-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8607908 Medline TA: J Control Release Country: Netherlands |
Other Details:
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Languages: eng Pagination: 279-88 Citation Subset: IM |
Affiliation:
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Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmacy, Ghent University, Harelbekestraat 72, B-9000 Ghent, Belgium. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Active Transport, Cell Nucleus Cell Line, Tumor Cell Membrane / metabolism* Cyclohexanols / metabolism DNA / metabolism* Humans Microbubbles* Nanoparticles* Nuclear Envelope / metabolism* Nuclear Pore / metabolism Phospholipids / metabolism Polyethylene Glycols / metabolism Transfection / methods* Ultrasonics* |
| Chemical | |
Reg. No./Substance:
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0/Cyclohexanols; 0/Phospholipids; 0/Polyethylene Glycols; 9007-49-2/DNA; 931-17-9/1,2-cyclohexanediol |
| Comments/Corrections | |
Comment In:
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J Control Release. 2008 Dec 18;132(3):151
[PMID:
18996157
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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