Document Detail


New strategies for echocardiographic evaluation of left ventricular function in a mouse model of long-term myocardial infarction.
MedLine Citation:
PMID:  22848568     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The aim of this article is to present an optimized acquisition and analysis protocol for the echocardiographic evaluation of left ventricle (LV) remodeling in a mouse model of myocardial infarction (MI).
METHODOLOGY: 13 female DBA/2J mice underwent permanent occlusion of the left anterior descending (LAD) coronary artery leading to MI. Mice echocardiography was performed using a Vevo 770 (Visualsonics, Canada) before infarction, and 7, 14, 30, 60, 90 and 120 days after LAD ligation. LV systolic function was evaluated using different parameters, including the fractional area change (FAC%) computed in four high-temporal resolution B-mode short axis images taken at different ventricular levels, and in one parasternal long axis. Pulsed wave and tissue Doppler modes were used to evaluate the diastolic function and Tei Index for global cardiac function. The echocardiographic measurements of infarct size were validated histologically using collagen deposition labeled by Sirius red staining. All data was analyzed using Shapiro-Wilk and Student's t-tests.
PRINCIPAL FINDINGS: Our results reveal LV dilation resulting in marked remodeling an severe systolic dysfunction, starting seven days after MI (LV internal apical diameter, basal = 2.82±0.24, 7d = 3.49±0.42; p<0.001. End-diastolic area, basal = 18.98±1.81, 7d = 22.04±2.11; p<0.001). A strong statistically significant negative correlation exists between the infarct size and long-axis FAC% (r = -0.946; R(2) = 0.90; p<0.05). Moreover, the measured Tei Index values confirmed significant post-infarction impairment of the global cardiac function (basal = 0.46±0.07, 7d = 0.55±0.08, 14 d = 0.57±0.06, 30 d = 0.54±0.06, 60 d = 0.54±0.07, 90 d = 0.57±0.08; p<0.01).
CONCLUSIONS/SIGNIFICANCE: In summary, we have performed a complete characterization of LV post-infarction remodeling in a DBA/2J mouse model of MI, using parameters adapted to the particular characteristics of the model In the future, this well characterized model will be used in both investigative and pharmacological studies that require accurate quantitative monitoring of cardiac recovery after myocardial infarction.
Authors:
Carolina Benavides-Vallve; David Corbacho; Olalla Iglesias-Garcia; Beatriz Pelacho; Edurne Albiasu; Sara Castaño; Arrate Muñoz-Barrutia; Felipe Prosper; Carlos Ortiz-de-Solorzano
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-07-27
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-07-31     Completed Date:  2013-04-09     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e41691     Citation Subset:  IM    
Affiliation:
Imaging Unit, Fundación para la Investigación Médica Aplicada, University of Navarra, Pamplona, Navarra, Spain.
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MeSH Terms
Descriptor/Qualifier:
Animals
Disease Models, Animal
Echocardiography / methods*
Female
Image Processing, Computer-Assisted
Mice
Myocardial Infarction / pathology,  physiopathology*,  ultrasonography*
Time Factors
Ventricular Function, Left / physiology*
Ventricular Remodeling
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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