Document Detail

New strategies for drug discovery: activation of silent or weakly expressed microbial gene clusters.
MedLine Citation:
PMID:  23143535     Owner:  NLM     Status:  MEDLINE    
Genome sequencing of Streptomyces, myxobacteria, and fungi showed that although each strain contains genes that encode the enzymes to synthesize a plethora of potential secondary metabolites, only a fraction are expressed during fermentation. Interest has therefore grown in the activation of these cryptic pathways. We review current progress on this topic, describing concepts for activating silent genes, utilization of "natural" mutant-type RNA polymerases and rare earth elements, and the applicability of ribosome engineering to myxobacteria and fungi, the microbial groups known as excellent searching sources, as well as actinomycetes, for secondary metabolites.
Kozo Ochi; Takeshi Hosaka
Related Documents :
24951565 - Family size evolution in drosophila chemosensory gene families: a comparative analysis ...
24828675 - Whole blood gene expression profile associated with spontaneous preterm birth in women ...
23936085 - Autotaxin signaling governs phenotypic heterogeneity in visceral and parietal mesothelia.
24111955 - Species-specific evolution of mhc class i genes in the higher primates.
18361395 - Kinetics of pronuclear development and the effects of vector type and timing of injecti...
19653105 - Overexpression of a rice gene encoding a small c2 domain protein ossmcp1 increases tole...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2012-11-11
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  97     ISSN:  1432-0614     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-04     Completed Date:  2013-06-04     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  87-98     Citation Subset:  IM    
Department of Life Science, Hiroshima Institute of Technology, Miyake 2-1-1, Saeki-ku, Hiroshima, 731-5193, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Bacteria / genetics,  metabolism
Biological Agents / isolation & purification*,  metabolism*
DNA-Directed RNA Polymerases / genetics,  metabolism
Drug Discovery / methods*,  trends
Fungi / genetics,  metabolism
Metabolic Engineering / methods
Metals, Rare Earth / metabolism
Multigene Family*
Ribosomes / genetics,  metabolism
Transcriptional Activation*
Reg. No./Substance:
0/Biological Agents; 0/Metals, Rare Earth; EC RNA Polymerases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  In vivo random mutagenesis of streptomycetes using mariner-based transposon Himar1.
Next Document:  Role of F-18 FDG PET/CT in assessing bone marrow involvement in pediatric Hodgkin's lymphoma.