Document Detail


New strategies for drug discovery: activation of silent or weakly expressed microbial gene clusters.
MedLine Citation:
PMID:  23143535     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Genome sequencing of Streptomyces, myxobacteria, and fungi showed that although each strain contains genes that encode the enzymes to synthesize a plethora of potential secondary metabolites, only a fraction are expressed during fermentation. Interest has therefore grown in the activation of these cryptic pathways. We review current progress on this topic, describing concepts for activating silent genes, utilization of "natural" mutant-type RNA polymerases and rare earth elements, and the applicability of ribosome engineering to myxobacteria and fungi, the microbial groups known as excellent searching sources, as well as actinomycetes, for secondary metabolites.
Authors:
Kozo Ochi; Takeshi Hosaka
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2012-11-11
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  97     ISSN:  1432-0614     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-04     Completed Date:  2013-06-04     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  87-98     Citation Subset:  IM    
Affiliation:
Department of Life Science, Hiroshima Institute of Technology, Miyake 2-1-1, Saeki-ku, Hiroshima, 731-5193, Japan. k.ochi.bz@it-hiroshima.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Bacteria / genetics,  metabolism
Biological Agents / isolation & purification*,  metabolism*
DNA-Directed RNA Polymerases / genetics,  metabolism
Drug Discovery / methods*,  trends
Fungi / genetics,  metabolism
Metabolic Engineering / methods
Metals, Rare Earth / metabolism
Multigene Family*
Ribosomes / genetics,  metabolism
Transcriptional Activation*
Chemical
Reg. No./Substance:
0/Biological Agents; 0/Metals, Rare Earth; EC 2.7.7.6/DNA-Directed RNA Polymerases
Comments/Corrections

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