Document Detail


New sialyl-Lewis-X analogues antagonize leukocyte-induced ischemia-reperfusion arrhythmia--a mapping study.
MedLine Citation:
PMID:  15103583     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Arrhythmia during ischemia and reperfusion is still an intriguing problem in cardiovascular medicine. Leukocytes infiltrating the ischemic region play an important pathophysiological role. The effects of soluble sialyl-Lewis-X analogues Hoe934553 and Hoe943644, which may inhibit leukocyte-endothelial interaction, were investigated. METHODS: Isolated rabbit hearts were perfused with Tyrode solution according to the Langendorff technique. Polymorphic neutrophilic granulocytes (PMN) were isolated from autologous peripheral blood. After 60 min equilibration PMN (n = 7) or vehicle (n = 7) were infused with or without concomitant treatment with Hoe934553 (n = 6) and Hoe943644 (n = 6). Five minutes after the start of the PMN infusion the left descending coronary artery was occluded for 30 min followed by 30 min of reperfusion. Activation and repolarization waves were recorded at 256 sites using a computerized mapping system. RESULTS: Ventricular fibrillation (VF) in 4/7 PMN-treated hearts was found, while in PMN-free hearts no VF occurred. Treatment with Hoe934553 and Hoe943644 completely prevented VF. PMN largely enhanced the dispersion of action potential duration during reperfusion. This PMN effect was completely prevented by both drugs. Myeloperoxidase assay showed reduced activity in Hoe934553 and Hoe943644 treated hearts. CONCLUSIONS: Sialyl-Lewis-X analogues (Hoe934553, Hoe943644) can antagonize PMN infiltration and PMN-induced VF in the course of ischemia and reperfusion.
Authors:
S Dhein
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Thoracic and cardiovascular surgeon     Volume:  52     ISSN:  0171-6425     ISO Abbreviation:  Thorac Cardiovasc Surg     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-04-22     Completed Date:  2004-11-09     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7903387     Medline TA:  Thorac Cardiovasc Surg     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  102-9     Citation Subset:  IM    
Affiliation:
Klinik für Herzchirurgie, Universität Leipzig, Herzzentrum, Leipzig, Germany. dhes@medizin.uni-leipzig.de
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MeSH Terms
Descriptor/Qualifier:
Animals
Arrhythmias, Cardiac / etiology*,  physiopathology
Coronary Circulation / physiology
Disease Models, Animal
Electrophysiologic Techniques, Cardiac
Heart Conduction System / cytology,  drug effects,  physiopathology
Heart Ventricles / cytology,  drug effects,  physiopathology
Leukocytes / drug effects*,  enzymology
Male
Models, Cardiovascular
Oligosaccharides / antagonists & inhibitors*
Peroxidase / drug effects
Rabbits
Reperfusion Injury / etiology*,  physiopathology
Time Factors
Ventricular Pressure / physiology
Chemical
Reg. No./Substance:
0/5-acetylneuraminyl-(2-3)-galactosyl-(1-4)-(fucopyranosyl-(1-3))-N-acetylglucosamine; 0/Oligosaccharides; EC 1.11.1.7/Peroxidase

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