| New role of antibody in bacterial isolation. | |
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MedLine Citation:
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PMID: 22468362 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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To eliminate the interference caused by Pseudomonas aeruginosa in the isolation of Salmonella, a rabbit polyclonal antibody against P. aeruginosa was prepared by inoculating four New Zealand rabbits with the pathogen. The antiserum was purified using saturated ammonium sulfate and added into Rappaport-Vassiliadis medium with soya (RVS) broth and Muller-Kauffmann tetrathionate novobiocin broth (MKTTn broth) to evaluate whether it could inhibit the growth of P. aeruginosa. Observations by scanning electron microscopy demonstrated that P. aeruginosa was attacked and destroyed by the antibody when incubated for 10 min at 37 degrees C. The activity of the antibody was also effective against 11 other strains of P. aeruginosa. Twenty-six strains of Salmonella were mixed with P. aeruginosa in RVS and MKTTn broth at 37 degrees C for 12 h, respectively, and the cultures were plated on Salmonella chromogenic medium (SCM; Oxoid, Basingstoke, UK). Only Salmonella grew on SCM; five colonies were randomly selected for identification by VITEK 2 (bioMérieux, Lyon, France). Additionally, when mixed with two strains of Enterobacter cloacae (ATCC 700323 and YG001), the prepared antibody did not affect the growth of E. cloacae. The results demonstrated that the microbicidal activity of the antibody did not affect the tested Salmonella sp. or E. cloacae strains. Therefore, the antibody generated could be used to increase the accuracy of Salmonella isolation. |
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Authors:
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Xizhi Xiao; Yu Chen; Mingjun Deng; Hongwei Gao; Zhenxing Wu; Laihua Zhu; Fei Yuan; Biao Xu; Chengzhu Liang; Yanming Zhang |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of AOAC International Volume: 95 ISSN: 1060-3271 ISO Abbreviation: J AOAC Int Publication Date: 2012 Jan-Feb |
Date Detail:
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Created Date: 2012-04-03 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9215446 Medline TA: J AOAC Int Country: United States |
Other Details:
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Languages: eng Pagination: 216-21 Citation Subset: IM |
Affiliation:
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Northwest A & F University, College of Veterinary Medicine, Yangling, Shaanxi, People's Republic of China 712100. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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