| New quantitative electrochemiluminescence method (ECLIA) for interleukin-6 (IL-6) measurement. | |
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MedLine Citation:
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PMID: 20298137 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: A new interleukin-6 (IL-6) electrochemiluminescent immunoassay (ECLIA, Roche Diagnostics) was evaluated and compared to a previous semiquantitative immunoassay (Milenia Biotec). Reference ranges for cord blood plasma were also calculated. METHODS: The new IL-6 ECLIA test was performed using a Cobas E601 analyzer (Roche Diagnostics). The comparison method (Milenia Biotec) was a semiquantitative lateral flow immunoassay, coupled to a digital image capture system (PicoScan). Healthy at term newborns were recruited to establish reference ranges for IL-6 in cord blood plasma. RESULTS: Total imprecision ranged from 3.7% to 8.0% depending on the IL-6 concentrations. The calculated limit of detection for IL-6 measured by ECLIA was 2.63 pg/mL, almost twice as high as that claimed by the manufacturer (1.5 pg/mL). The linearity of the method was verified to 5000 pg/mL. The IL-6 reference limit, obtained from 148 cord blood samples, was 30 pg/mL (90% confidence interval: 19-48 pg/mL). CONCLUSIONS: The new quantitative ECLIA method showed good reproducibility, linearity and functional sensitivity. Additional clinically based studies are needed to elucidate the usefulness of the IL-6 reference limit, calculated here for the first time by ECLIA in cord blood plasma, to aid in the diagnosis of vertical transmission of sepsis. |
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Authors:
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Belén Prieto; Diego Miguel; Marta Costa; Daniel Coto; Francisco V Alvarez |
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Publication Detail:
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Type: Comparative Study; Evaluation Studies; Journal Article |
Journal Detail:
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Title: Clinical chemistry and laboratory medicine : CCLM / FESCC Volume: 48 ISSN: 1434-6621 ISO Abbreviation: Clin. Chem. Lab. Med. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-06-03 Completed Date: 2010-09-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9806306 Medline TA: Clin Chem Lab Med Country: Germany |
Other Details:
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Languages: eng Pagination: 835-8 Citation Subset: IM |
Affiliation:
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Clinical Biochemistry Laboratory, Hospital Universitario Central de Asturias, Oviedo, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Chemiluminescent Measurements
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methods* Fetal Blood Humans Immunoassay / methods Infant, Newborn Interleukin-6 / blood* Reagent Kits, Diagnostic Reproducibility of Results Sepsis / diagnosis |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-6; 0/Reagent Kits, Diagnostic |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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