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New procedure using a psoralen derivative for analysis of nucleosome associated DNA sequences in chromatin of living cells.
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MedLine Citation:
PMID:  6281729     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sites for restriction endonuclease cleavage in double helical DNA are blocked from cleavage when the photoaffinity drug trimethylpsoralen is photobound at or near the site. In general, Hind III sites are about 15 fold more sensitive to inactivation than the other restriction sites which were tested, although sensitivity of different Hind III sites seems to vary somewhat depending on base sequences adjacent to the site. Hind III sites can be inactivated in two ways; one which completely blocks action of the specific restriction endonuclease and one permitting the introduction of a swivel which relaxes DNA supercoiling without producing a double strand break. Nucleosomes and perhaps other protein-DNA complexes can protect the underlying DNA sequence from trimethylpsoralen photobinding and thus protect restriction sites from inactivation. This property can be exploited to determine if specific sites are accessible to the psoralon probe in vivo and thus to establish if specific nucleotide sequences are nucleosome associated. Using this procedure evidence is obtained that nucleosomes on SV40 DNA in living infected cells are either distributed randomly or at many discrete alternate sites that approach a random distribution.
Authors:
J O Carlson; O Pfenninger; R R Sinden; J M Lehman; D E Pettijohn
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Nucleic acids research     Volume:  10     ISSN:  0305-1048     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  1982 Mar 
Date Detail:
Created Date:  1982-07-08     Completed Date:  1982-07-08     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2043-63     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cercopithecus aethiops
Chromatin / analysis*
DNA Restriction Enzymes / metabolism
DNA, Viral / genetics*
Genes, Viral
Kinetics
Nucleic Acid Conformation
Nucleosomes / analysis*
Psoralens* / pharmacology
Simian virus 40 / genetics
Trioxsalen* / pharmacology
Grant Support
ID/Acronym/Agency:
GM 1824311/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Chromatin; 0/DNA, Viral; 0/Nucleosomes; 0/Psoralens; 3902-71-4/Trioxsalen; EC 3.1.21.-/DNA Restriction Enzymes
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Nucleic Acids Res
ISSN: 0305-1048
ISSN: 1362-4962
Article Information
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Print publication date: Day: 25 Month: 3 Year: 1982
Volume: 10 Issue: 6
First Page: 2043 Last Page: 2063
ID: 320588
PubMed Id: 6281729

New procedure using a psoralen derivative for analysis of nucleosome associated DNA sequences in chromatin of living cells.
J O Carlson
O Pfenninger
R R Sinden
J M Lehman
D E Pettijohn



Article Categories:
  • Research Article


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