Document Detail

New procedure using a psoralen derivative for analysis of nucleosome associated DNA sequences in chromatin of living cells.
Jump to Full Text
MedLine Citation:
PMID:  6281729     Owner:  NLM     Status:  MEDLINE    
Sites for restriction endonuclease cleavage in double helical DNA are blocked from cleavage when the photoaffinity drug trimethylpsoralen is photobound at or near the site. In general, Hind III sites are about 15 fold more sensitive to inactivation than the other restriction sites which were tested, although sensitivity of different Hind III sites seems to vary somewhat depending on base sequences adjacent to the site. Hind III sites can be inactivated in two ways; one which completely blocks action of the specific restriction endonuclease and one permitting the introduction of a swivel which relaxes DNA supercoiling without producing a double strand break. Nucleosomes and perhaps other protein-DNA complexes can protect the underlying DNA sequence from trimethylpsoralen photobinding and thus protect restriction sites from inactivation. This property can be exploited to determine if specific sites are accessible to the psoralon probe in vivo and thus to establish if specific nucleotide sequences are nucleosome associated. Using this procedure evidence is obtained that nucleosomes on SV40 DNA in living infected cells are either distributed randomly or at many discrete alternate sites that approach a random distribution.
J O Carlson; O Pfenninger; R R Sinden; J M Lehman; D E Pettijohn
Related Documents :
24950479 - Engineering cellulosic bioreactors by template assisted dna shuffling.
7663399 - In vitro proteolysis of endonucleases in rat liver nuclei extracts.
2985469 - Recognition sequences of restriction endonucleases and methylases--a review.
24965879 - Development of a new dumbbell-shaped decoy dna using a combination of the unnatural bas...
12665299 - Iodouracil-mediated photocrosslinking of dna to ecorii restriction endonuclease in cata...
23335959 - The effect of a g:t mispair on the dynamics of dna.
24170979 - Pluchea lanceolata protects against benzo(a) pyrene induced renal toxicity and loss of ...
24569169 - Single molecule approaches to monitor the recognition and resection of double-stranded ...
8666999 - The roles of cre, tre, and tre-adjacent s1 nuclease sensitive element in the regulation...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Nucleic acids research     Volume:  10     ISSN:  0305-1048     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  1982 Mar 
Date Detail:
Created Date:  1982-07-08     Completed Date:  1982-07-08     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2043-63     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Line
Cercopithecus aethiops
Chromatin / analysis*
DNA Restriction Enzymes / metabolism
DNA, Viral / genetics*
Genes, Viral
Nucleic Acid Conformation
Nucleosomes / analysis*
Psoralens* / pharmacology
Simian virus 40 / genetics
Trioxsalen* / pharmacology
Grant Support
Reg. No./Substance:
0/Chromatin; 0/DNA, Viral; 0/Nucleosomes; 0/Psoralens; 3902-71-4/Trioxsalen; EC 3.1.21.-/DNA Restriction Enzymes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Nucleic Acids Res
ISSN: 0305-1048
ISSN: 1362-4962
Article Information
Download PDF

Print publication date: Day: 25 Month: 3 Year: 1982
Volume: 10 Issue: 6
First Page: 2043 Last Page: 2063
ID: 320588
PubMed Id: 6281729

New procedure using a psoralen derivative for analysis of nucleosome associated DNA sequences in chromatin of living cells.
J O Carlson
O Pfenninger
R R Sinden
J M Lehman
D E Pettijohn

Article Categories:
  • Research Article

Previous Document:  Isolation and characterization of a human collagen alpha 1(I)-like gene from a cosmid library.
Next Document:  New rapid methods for DNA sequencing based in exonuclease III digestion followed by repair synthesis...