| New polymeric benzotriazole reagents for off-line derivatizations of amines and polyamines in HPLC. | |
| | |
MedLine Citation:
|
PMID: 3225308 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
New polymeric reagents are synthesized, based on a polystyrene-bound benzotriazole containing an o-acetylsalicyl or 9-fluorenyl labelling moiety. This is used in an off-line mode, prior to high performance liquid chromatography (HPLC), for derivatizations of trace primary and secondary amines, polyamines, and related compounds in connection with HPLC. Standards are prepared, characterized by physical and spectral properties, and then used as external standards to determine percent derivatizations. The polymeric reagents are characterized by elemental analyses and loading determinations. The feasibility and applicability of this reagent for derivatization of nucleophiles is confirmed with a number of amines under optimized conditions. The activated labelling moiety, bonded to the polymeric support, makes the derivatization reactions extremely rapid and efficient under mild reaction conditions. This alone provides significant advantages over the analogous solution derivatizations for the same amines. A comparison of solution and solid phase derivatization reactions is reported. The limits of detection are 1 to 2 pmol for polyamines, such as cadaverine, putrescine, and 1,7-diaminoheptane, using the benzotriazole fluorenyl reagent followed by fluorescence detection. |
| | |
Authors:
|
C X Gao; T Y Chou; S T Colgan; I S Krull; C Dorschel; B Bidlingmeyer |
Related Documents
:
|
20819278 - Determination of amines in the presence of excess ammonia by ion chromatography-mass sp... 19882628 - Sensitive profiling of biogenic amines in urine using ce with transient isotachophoreti... 19364488 - Determination of dansylated polyamines in red blood cells by liquid chromatography-tand... 1400708 - Drug level monitoring: antidepressants. 20663038 - Biological activity of the essential oil of kadsura longipedunculata (schisandraceae) a... 15044678 - Pleural mechanics and fluid exchange. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of chromatographic science Volume: 26 ISSN: 0021-9665 ISO Abbreviation: J Chromatogr Sci Publication Date: 1988 Sep |
Date Detail:
|
Created Date: 1989-03-28 Completed Date: 1989-03-28 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0173225 Medline TA: J Chromatogr Sci Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 449-57 Citation Subset: IM |
Affiliation:
|
Department of Chemistry, Barnett Institute, Northeastern University, Boston, Massachusetts 02115. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amines
/
analysis* Chromatography, High Pressure Liquid Indicators and Reagents Polyamines / analysis* Solvents Triazoles* |
| Chemical | |
Reg. No./Substance:
|
0/Amines; 0/Indicators and Reagents; 0/Polyamines; 0/Solvents; 0/Triazoles; 27556-51-0/benzotriazole |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: C18 solid-phase isolation and high-performance liquid chromatography/ultraviolet diode array determi...
Next Document: Determination of menadione in an animal feed using supercritical fluid extraction and HPLC with elec...