Document Detail

New plasma membrane proteins expressed by a high metastatic variant of a chemically induced tumor.
MedLine Citation:
PMID:  6332734     Owner:  NLM     Status:  MEDLINE    
The composition of plasma membrane proteins of related low and high metastatic tumor cells were compared in order to elucidate possible genetic changes which may occur during progression of tumor cells towards metastatic capacity. For this purpose a procedure was worked out which allows the purification of mouse plasma membrane (PM) proteins from biosynthetically labeled cells. PM isolated from the two related T lymphoma lines Eb and ESb, which differ greatly in metastatic capacity, as well as from Con A-activated T cell blasts displayed similar degrees of purification as shown by marker enzyme analysis. When [35S]-methionine-labeled proteins of such PM preparations from cloned Eb and ESb cells were compared by 2-D gel electrophoresis it was found that maps of the highly metastatic variant ESb cells permitted detection of about 50 new protein spots which were not found on the 2-D gel maps of the parental Eb cells. Approximately 80% of these new spots were also found on maps derived from syngeneic splenic Con A blasts. This observation indicated that most of the newly expressed membrane proteins of ESb cells were possible differentiation or cell activation-related and not tumor cell-specific.
P Altevogt; V Schirrmacher
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of cancer & clinical oncology     Volume:  20     ISSN:  0277-5379     ISO Abbreviation:  Eur J Cancer Clin Oncol     Publication Date:  1984 Sep 
Date Detail:
Created Date:  1984-11-05     Completed Date:  1984-11-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8112045     Medline TA:  Eur J Cancer Clin Oncol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1155-62     Citation Subset:  IM    
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MeSH Terms
Centrifugation, Density Gradient
Electrophoresis, Polyacrylamide Gel
Isoelectric Focusing
Leukemia L5178 / analysis,  chemically induced,  metabolism*
Leukemia, Experimental / metabolism*
Lymphocyte Activation
Membrane Proteins / analysis*
Mice, Inbred DBA
Neoplasm Proteins / analysis
T-Lymphocytes / analysis
Reg. No./Substance:
0/Membrane Proteins; 0/Neoplasm Proteins; 56-49-5/Methylcholanthrene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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