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A NEW NANO-ENHANCED TECHNOLOGY PROPOSED TO QUANTIFY INTRACELLULAR DETECTION OF RADIATION-INDUCED METABOLIC PROCESSES.
MedLine Citation:
PMID:  21196462     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A new approach to intracellular detection and imaging of metabolic processes and pathways is presented that uses surface plasmon resonance to enhance interactions between photon-absorbing metabolites and metal nanoparticles in contact with cells in vitro or in vivo. Photon absorption in the nanoparticles creates plasmon fields, enhancing intrinsic metabolite fluorescence, thereby increasing absorption and emission rates, creating new spectral emission bands, shortening fluorescence lifetimes, becoming more photo-stable and increasing fluorescent resonance energy transfer efficiency. Because the cells remain viable, it is proposed that the method may be used to interrogate cells prior to and after irradiation, with the potential for automated analyses of intracellular interactive pathways associated with radiation exposures at lower doses than existing technologies. The design and concepts of the instrument are presented along with data for unexposed cells.
Authors:
Henryk Malak; Robert Richmond; J F Dicello
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2010-12-31
Journal Detail:
Title:  Radiation protection dosimetry     Volume:  -     ISSN:  1742-3406     ISO Abbreviation:  -     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2011-1-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8109958     Medline TA:  Radiat Prot Dosimetry     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
American Environmental Systems, Inc., 8444 High Ridge Road, Ellicott City, MD 21043, USA.
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