Document Detail


New mutant versions of yeast FACT subunit Spt16 affect cell integrity.
MedLine Citation:
PMID:  19727824     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Transcription by RNA polymerase II is impeded by the nucleosomal organization of DNA; these negative effects are modulated at several stages of nucleosomal DNA transcription by FACT, a heterodimeric transcription factor. At promoters, FACT facilitates the binding of TATA-binding factor, while during transcription elongation FACT mediates the necessary destabilization of nucleosomes and subsequent restoration of nucleosome structure in the wake of the transcription elongation complex. Altered FACT activity can impair the fidelity of transcription initiation and affect transcription patterns. Using reporter genes we have identified new mutant versions of the Spt16 subunit of yeast FACT with dominant negative effects on the fidelity of transcription initiation. Two of these spt16 mutant alleles also affect cell integrity. Cells relying on these spt16 mutant alleles display sorbitol-remediated temperature sensitivity, altered sensitivity to detergent, and abnormal morphologies, and are further inhibited by the ssd1-d mutation. The overexpression of components of protein kinase C (Pkc1) signaling diminishes this spt16 ssd1-d temperature sensitivity, whereas gene deletions eliminating components of Pkc1 signaling further impair these spt16 mutant cells. Thus, the FACT subunit Spt16 and Pkc1 signaling have an overlapping essential function, with an unexpected role for FACT in the maintenance of cell integrity.
Authors:
Allyson F O'Donnell; Jennifer R Stevens; Rosemarie Kepkay; Christine A Barnes; Gerald C Johnston; Richard A Singer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-01
Journal Detail:
Title:  Molecular genetics and genomics : MGG     Volume:  282     ISSN:  1617-4623     ISO Abbreviation:  Mol. Genet. Genomics     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2010-01-29     Completed Date:  2010-02-22     Revised Date:  2012-06-22    
Medline Journal Info:
Nlm Unique ID:  101093320     Medline TA:  Mol Genet Genomics     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  487-502     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Biology, Dalhousie University, Sir Charles Tupper Medical Building, 5850 College Street, Halifax, NS, B3H 1X5, Canada.
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MeSH Terms
Descriptor/Qualifier:
Alleles
Cell Wall / drug effects,  genetics
Gene Expression Regulation, Fungal / drug effects
Genes, Dominant / genetics
Genes, Fungal / genetics
Genes, Reporter
Genetic Complementation Test
Hydroxyurea / pharmacology
Mutation / genetics*
Phenotype
Protein Kinase C / genetics,  metabolism
Protein Subunits / genetics*
RNA, Messenger / genetics,  metabolism
Saccharomyces cerevisiae / cytology*,  drug effects,  genetics*
Saccharomyces cerevisiae Proteins / genetics*,  metabolism
Signal Transduction / drug effects,  genetics
Stress, Physiological / drug effects,  genetics
Suppression, Genetic / drug effects
Temperature
Transcription, Genetic / drug effects
Transcriptional Elongation Factors / genetics*,  metabolism
beta-Galactosidase / metabolism
Grant Support
ID/Acronym/Agency:
//Canadian Institutes of Health Research
Chemical
Reg. No./Substance:
0/Protein Subunits; 0/RNA, Messenger; 0/SPT16 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; 0/Transcriptional Elongation Factors; 127-07-1/Hydroxyurea; EC 2.7.11.13/PKC1 protein, S cerevisiae; EC 2.7.11.13/Protein Kinase C; EC 3.2.1.23/beta-Galactosidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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