Document Detail


New mini-ColE1 as a molecular cloning vehicle.
MedLine Citation:
PMID:  318644     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new mini-ColE1 plasmid, designated pAC105, was isolated. It has a molecular weight of 1.6 X 10(6) and carries information for its self-replication as well as information for conferring colicin E1 immunity upon its host. Furthermore, pAC105 undergoes replication in the presence of chloramphenicol even when a foreign deoxyribonucleic acid (pSC101) is inserted into its single EcoRI restriction site. Studies in minicell-producing strains demonstrate that pAC105 codes for only two or three polypeptides of low molecular weight. The advantages of using it as a molecular cloning vehicle are discussed.
Authors:
H Avni; P E Berg; A Markovitz
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  129     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1977 Jan 
Date Detail:
Created Date:  1977-02-26     Completed Date:  1977-02-26     Revised Date:  2010-09-02    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  358-66     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / biosynthesis
Chloramphenicol / pharmacology
Colicins / pharmacology*
DNA, Bacterial / biosynthesis
DNA, Circular / biosynthesis
Drug Resistance, Microbial
Escherichia coli* / drug effects
Extrachromosomal Inheritance*
Genes*
Plasmids*
Rifampin / pharmacology
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Colicins; 0/DNA, Bacterial; 0/DNA, Circular; 13292-46-1/Rifampin; 56-75-7/Chloramphenicol
Comments/Corrections

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