Document Detail


New method for manipulation, maintenance, and cloning of single mammalian cells in vitro.
MedLine Citation:
PMID:  13794486     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Individual mammalian cells can be isolated with the aid of easily handled glass beads to which the cells have become attached. Procedures for the preparation of cell cultures on beads, and for the recognition and manipulation of beads carrying single monocytes, HeLa cells, and Detroit-98 cells are described. Data obtained with Brucella infected monocytes, illustrating the efficiency of the method, are presented.
Authors:
M AROSON; R W KESSEL
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  131     ISSN:  0036-8075     ISO Abbreviation:  Science     Publication Date:  1960 May 
Date Detail:
Created Date:  1960-12-01     Completed Date:  1998-11-01     Revised Date:  2011-12-02    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  Not Available    
Other Details:
Languages:  eng     Pagination:  1376-7     Citation Subset:  OM    
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MeSH Terms
Descriptor/Qualifier:
Tissue Culture Techniques*

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