Document Detail


New method for isolating antibiotic-producing fungi.
MedLine Citation:
PMID:  23149518     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A convenient and efficient method was established for isolating antifungal antibiotic-producing fungi from soil samples. In this method, soil samples were diluted and directly plated in agar medium by the standard fungi-isolating method, and the plates were cultured at 27 °C for 2-3 days to permit the growth of fungal colonies. Then, the suspension of pathogenic Candida albicans in saline (40 μl, 5-10 × 10(5) CFU ml(-1)) was overlaid by spraying on the plates under controlled conditions in the safety cabinet. After 1-day incubation, fungal colonies showing an antagonistic effect with the inhibition zone against sprayed C. albicans were selected. Among 151 isolates, 26 strains were found to reproduce anti-C. albicans activity in liquid medium, yielding a higher selection rate (17.2%) than that (3.1%) by the traditional method. This new method can be applied for isolation of microorganisms (fungi and actinomycetes) that produce antibiotics active against pathogenic microorganisms.The Journal of Antibiotics advance online publication, 14 November 2012; doi:10.1038/ja.2012.79.
Authors:
Mio Kawaguchi; Kenichi Nonaka; Rokuro Masuma; Hiroshi Tomoda
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-14
Journal Detail:
Title:  The Journal of antibiotics     Volume:  -     ISSN:  0021-8820     ISO Abbreviation:  J. Antibiot.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0151115     Medline TA:  J Antibiot (Tokyo)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Graduate School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
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