| A NEW METHOD FOR FOCAL TRANSIENT CEREBRAL ISCHAEMIA BY DISTAL COMPRESSION OF THE MIDDLE CEREBRAL ARTERY. | |
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MedLine Citation:
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PMID: 22289071 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Aims: Rodent experimental models are essential for in vivo study of stroke. Our aim was to develop a reproducible method of mouse transient focal cerebral ischaemia by distal artery compression. Methods: The distal middle cerebral artery (dMCA) was occluded by compression with a blunted needle and cerebral blood flow was monitored by Laser Doppler Flowmetry (LDF) to ensure appropriate occlusion and reperfusion in Balb/c mice. The ischaemic lesion was evaluated 24 hours after occlusion by TTC staining and immunolabeling (NeuN, CD31, GFAP and Iba-1) while the established permanent dMCA occlusion model was used as a control. The corner test was performed to evaluate neurological behaviour. Results: LDF-register showed a homogenous arterial occlusion among animals. Forty-five minutes of arterial occlusion did not lead brain infarction when evaluated by TTC staining 24 hours after occlusion. Extending the cerebral ischaemia period to 60 minutes induced a cortically localized homogeneous brain infarct. No differences in infarct volume were detected between animals submitted to permanent or 60 minutes transient dMCAO (42.33 ± 9.88 mm(3) and 37.63 ± 12.09 mm(3) , respectively). The ischaemic injury was confirmed by immunohistochemistry in the 60 minutes transient dMCAO model but not in the 45 minutes model. Neurological deficits assessed with the corner test were significant only during the first 48 hours but not at long-term. Conclusions: This work shows an easy to perform method for the induction of brain ischaemia and reperfusion to assess stroke repair and treatment screening, with cortically localized ischaemic cell damage, low mortality and neurological impairment in the acute phase. © 2012 The Authors. Neuropathology and Applied Neurobiology © 2012 British Neuropathological Society. |
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Authors:
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Anna Morancho; Lidia García-Bonilla; Verónica Barceló; Dolors Giralt; Mireia Campos-Martorell; Sandra Garcia; Joan Montaner; Anna Rosell |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-30 |
Journal Detail:
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Title: Neuropathology and applied neurobiology Volume: - ISSN: 1365-2990 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-31 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7609829 Medline TA: Neuropathol Appl Neurobiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2012 The Authors. Neuropathology and Applied Neurobiology © 2012 British Neuropathological Society. |
Affiliation:
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Neurovascular Research Laboratory, Department of Neurology and Department of Internal Medicine, Universitat Autònoma de Barcelona. Vall d'Hebron Institut de Recerca, Barcelona, Spain. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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