| New method for the determination of pancreatic amylase evaluated. | |
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MedLine Citation:
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PMID: 1723097 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have carried out a multicenter evaluation of a new reagent carrier for Reflotron, specific for pancreatic amylase where the salivary isoenzyme is inhibited by two specific monoclonal antibodies. This new procedure combines easy handling with low imprecision (median CV less than 3%) in control material, serum, heparinized blood, and plasma) and close correlation (r = 0.991 to 0.999) with established manual and automated methods. The same close correlation was found with values obtained from either venous or capillary finger-stick blood. Salivary amylase up to 54 kU/L (37 degrees C) was inhibited to about 97%. Endogenous interference by hemoglobin, bilirubin, triglycerides, cholesterol, or hematocrit was found to be negligible within a wide range of interferent concentrations. Out of a panel of 28 commonly used drugs it was shown that only two (ascorbic acid and paracetamol), and then only at toxic concentrations, caused a deviation in amylase activity of greater than 10%. From the results of this study we conclude that this new method is suitable for highly precise and accurate measurements of pancreatic amylase in emergency and routine laboratories. |
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Authors:
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K Lorentz; R Bais; P M Bayer; H Keller; M Puukka; J Ruostesuo; R Puukka; S B Rosalki; Y Foo; D Seiler |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Journal of clinical laboratory analysis Volume: 5 ISSN: 0887-8013 ISO Abbreviation: J. Clin. Lab. Anal. Publication Date: 1991 |
Date Detail:
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Created Date: 1992-03-04 Completed Date: 1992-03-04 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8801384 Medline TA: J Clin Lab Anal Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 410-4 Citation Subset: IM |
Affiliation:
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Institut für Klinische Chemie, Medizinische Universität, Lübeck, FRG. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amylases
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analysis* Evaluation Studies as Topic Humans Indicators and Reagents Isoenzymes / analysis Pancreas / enzymology* Saliva / enzymology Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Indicators and Reagents; 0/Isoenzymes; EC 3.2.1.-/Amylases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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