Document Detail

New method for the determination of pancreatic amylase evaluated.
MedLine Citation:
PMID:  1723097     Owner:  NLM     Status:  MEDLINE    
We have carried out a multicenter evaluation of a new reagent carrier for Reflotron, specific for pancreatic amylase where the salivary isoenzyme is inhibited by two specific monoclonal antibodies. This new procedure combines easy handling with low imprecision (median CV less than 3%) in control material, serum, heparinized blood, and plasma) and close correlation (r = 0.991 to 0.999) with established manual and automated methods. The same close correlation was found with values obtained from either venous or capillary finger-stick blood. Salivary amylase up to 54 kU/L (37 degrees C) was inhibited to about 97%. Endogenous interference by hemoglobin, bilirubin, triglycerides, cholesterol, or hematocrit was found to be negligible within a wide range of interferent concentrations. Out of a panel of 28 commonly used drugs it was shown that only two (ascorbic acid and paracetamol), and then only at toxic concentrations, caused a deviation in amylase activity of greater than 10%. From the results of this study we conclude that this new method is suitable for highly precise and accurate measurements of pancreatic amylase in emergency and routine laboratories.
K Lorentz; R Bais; P M Bayer; H Keller; M Puukka; J Ruostesuo; R Puukka; S B Rosalki; Y Foo; D Seiler
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of clinical laboratory analysis     Volume:  5     ISSN:  0887-8013     ISO Abbreviation:  J. Clin. Lab. Anal.     Publication Date:  1991  
Date Detail:
Created Date:  1992-03-04     Completed Date:  1992-03-04     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8801384     Medline TA:  J Clin Lab Anal     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  410-4     Citation Subset:  IM    
Institut für Klinische Chemie, Medizinische Universität, Lübeck, FRG.
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MeSH Terms
Amylases / analysis*
Evaluation Studies as Topic
Indicators and Reagents
Isoenzymes / analysis
Pancreas / enzymology*
Saliva / enzymology
Sensitivity and Specificity
Reg. No./Substance:
0/Indicators and Reagents; 0/Isoenzymes; EC 3.2.1.-/Amylases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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