| New ligase-derived RNA polymerase ribozymes. | |
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MedLine Citation:
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PMID: 15987804 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The search is underway for a catalytic RNA molecule capable of self-replication. Finding such a ribozyme would lend crucial support to the RNA World hypothesis, which holds that very early life-forms relied on RNA for both replicating and storing genetic information. We previously reported an RNA polymerase isolated from a pool of variants of an existing RNA ligase ribozyme. Here we report eight additional ligase-derived polymerase ribozymes isolated from this pool. Because each of them is a new potential starting point for further in vitro evolution and engineering, together they substantially enrich the set of candidates from which an RNA replicase ribozyme might eventually emerge. |
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Authors:
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Michael S Lawrence; David P Bartel |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S. Date: 2005-06-29 |
Journal Detail:
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Title: RNA (New York, N.Y.) Volume: 11 ISSN: 1355-8382 ISO Abbreviation: RNA Publication Date: 2005 Aug |
Date Detail:
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Created Date: 2005-07-26 Completed Date: 2005-09-30 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 9509184 Medline TA: RNA Country: United States |
Other Details:
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Languages: eng Pagination: 1173-80 Citation Subset: IM |
Affiliation:
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Whitehead Institute for Biomedical Research/Biology Department, Massachusetts Institute of Technology, Cambridge, MA 02142, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Cloning, Molecular DNA-Directed RNA Polymerases / metabolism* Kinetics Molecular Sequence Data Nucleic Acid Conformation RNA / genetics, ultrastructure RNA Ligase (ATP) / metabolism* RNA, Catalytic / metabolism* Recombinant Proteins / metabolism |
| Chemical | |
Reg. No./Substance:
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0/RNA, Catalytic; 0/Recombinant Proteins; 63231-63-0/RNA; EC 2.7.7.6/DNA-Directed RNA Polymerases; EC 6.5.1.3/RNA Ligase (ATP) |
| Comments/Corrections | |
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