Document Detail


New horny layer marker proteins for evaluating skin condition in atopic dermatitis.
MedLine Citation:
PMID:  19339807     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Atopic dermatitis (AD) has a complicated pathogenesis and its clinical features vary greatly among patients. Although many clinical parameters have been reported, it remains difficult to evaluate AD skin conditions adequately.
OBJECTIVE: To support better evaluation of AD patients, we attempted to develop a new, objective and noninvasive method that assesses skin condition in AD using biochemical markers in the skin's horny layer (HL).
METHODS: Thirty-six patients with AD, 8 with psoriasis and 16 healthy volunteers were recruited. HL samples were obtained by tape stripping from involved and uninvolved skin of the forearms. Expression levels of 6 proteins in the HL [fatty acid-binding protein-5 (FABP-5), squamous cell carcinoma antigens 2 (SCCA2), alpha-enolase, annexin II, apolipoprotein A-I and albumin] were analyzed by immunoblotting and compared with clinical data.
RESULTS: The 6 proteins were detected at a high level in AD skin lesions, but scarcely in the normal controls. FABP-5 showed correlation with the local severity of the involved skin. Annexin II, apoprotein A-I and albumin showed correlation with the severity of specific eruptions. SCCA2 correlated significantly with total serum IgE level. Albumin levels in the uninvolved skin of AD patients showed significant correlation with the local severity in the involved skin of the same patient and with the trans-epidermal water loss. Albumin levels in psoriatic skin were very low, even with scratch marks, compared to those in AD skin.
CONCLUSION: FABP-5, albumin and some other proteins in HL seem to be useful as biomarkers to evaluate inflammation and skin barrier conditions in AD patients.
Authors:
Yumiko Yamane; Kayano Moriyama; Chie Yasuda; Satoshi Miyata; Michiko Aihara; Zenro Ikezawa; Kaoru Miyazaki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-04-02
Journal Detail:
Title:  International archives of allergy and immunology     Volume:  150     ISSN:  1423-0097     ISO Abbreviation:  Int. Arch. Allergy Immunol.     Publication Date:  2009  
Date Detail:
Created Date:  2009-08-17     Completed Date:  2009-09-04     Revised Date:  2014-07-30    
Medline Journal Info:
Nlm Unique ID:  9211652     Medline TA:  Int Arch Allergy Immunol     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  89-101     Citation Subset:  IM    
Copyright Information:
Copyright 2009 S. Karger AG, Basel.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Albumins / biosynthesis
Annexin A2 / biosynthesis
Antigens, Neoplasm / biosynthesis
Apolipoprotein A-I / biosynthesis
Biological Markers / analysis*
Blotting, Western
Child
Child, Preschool
Dermatitis, Atopic / metabolism*,  pathology*
Fatty Acid-Binding Proteins / biosynthesis
Female
Gene Expression*
Humans
Male
Middle Aged
Phosphopyruvate Hydratase / biosynthesis
Serpins / biosynthesis
Young Adult
Chemical
Reg. No./Substance:
0/Albumins; 0/Annexin A2; 0/Antigens, Neoplasm; 0/Apolipoprotein A-I; 0/Biological Markers; 0/FABP5 protein, human; 0/Fatty Acid-Binding Proteins; 0/Serpins; 0/squamous cell carcinoma-related antigen; EC 4.2.1.11/Phosphopyruvate Hydratase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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