Document Detail


New fluorescence reactions in DNA cytochemistry. 2. Microscopic and spectroscopic studies on fluorescent aluminum complexes.
MedLine Citation:
PMID:  2302307     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Metal-dye complexes are widely applied in light microscopic techniques for chromatin staining (e.g., hematoxylin and carmine), but fluorescent complexes between phosphate-binding cations and suitable ligands have been little used. Preformed and postformed Al complexes with different anionic dyes induced strong and selective fluorescence reactions in nuclei from chicken blood smears, frozen sections, paraffin-embedded sections and Epon-embedded sections of mouse and rat tissues, mitotic chromosomes, meiotic chromosomes and kinetoplasts of Trypanosoma cruzi epimastigotes. The DNA-dependent fluorescence of these structures showed a very low fading rate. The emission colors were related to the ligand. The most suitable compounds for forming fluorescent Al chelates were 8-hydroxyquinoline, morin, nuclear fast red and purpurin. Staining with diluted carmine solutions and InCl3 mordanting, followed by 8-hydroxyquinoline, also induced chromatin fluorescence. After treating isolated mouse chromosomes with the preformed complex Al-nuclear fast red, x-ray microanalysis indicated a P:Al:dye binding ratio of about 40:15:1. The selectivity, stability and easy formation of these fluorescent Al complexes are obvious advantages for their use as new cytochemical probes in cytologic studies.
Authors:
P Del Castillo; A R Llorente; A Gómez; J Gosálvez; V J Goyanes; J C Stockert
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology     Volume:  12     ISSN:  0884-6812     ISO Abbreviation:  Anal. Quant. Cytol. Histol.     Publication Date:  1990 Feb 
Date Detail:
Created Date:  1990-03-27     Completed Date:  1990-03-27     Revised Date:  2014-11-05    
Medline Journal Info:
Nlm Unique ID:  8506819     Medline TA:  Anal Quant Cytol Histol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  11-20     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aluminum / metabolism*
Animals
Chelating Agents / metabolism
Chromatin / metabolism
DNA / analysis*,  metabolism
Electron Probe Microanalysis
Fluorescent Dyes* / metabolism
Histocytochemistry / methods
Microscopy, Fluorescence / methods
Spectrometry, Fluorescence
Chemical
Reg. No./Substance:
0/Chelating Agents; 0/Chromatin; 0/Fluorescent Dyes; 9007-49-2/DNA; CPD4NFA903/Aluminum

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