Document Detail

New detection and separation method for amino acids by high-performance liquid chromatography.
MedLine Citation:
PMID:  7217249     Owner:  NLM     Status:  MEDLINE    
A simple system for simultaneous fluorescence detection of primary and secondary amines has been developed. The optimum conditions for fluorescing secondary amines in the two-stage reaction technique using sodium hypochlorite and o-phthalaldehyde have been studied, and then the switching flow method (sodium hypochlorite is added only to secondary amines) and the non-switching flow method (sodium hypochlorite is added to all the amino acids) have been compared. It was concluded that the non-switching flow method ensures high efficiency and reliability. This newly-developed system was applied to the detection of amino acids and gave satisfactory results.
Y Ishida; T Fujita; K Asai
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography     Volume:  204     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1981 Jan 
Date Detail:
Created Date:  1981-06-13     Completed Date:  1981-06-13     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  143-8     Citation Subset:  IM    
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MeSH Terms
Amino Acids / analysis*
Chromatography, High Pressure Liquid / methods*
Reg. No./Substance:
0/Amino Acids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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