| New detection and separation method for amino acids by high-performance liquid chromatography. | |
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MedLine Citation:
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PMID: 7217249 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A simple system for simultaneous fluorescence detection of primary and secondary amines has been developed. The optimum conditions for fluorescing secondary amines in the two-stage reaction technique using sodium hypochlorite and o-phthalaldehyde have been studied, and then the switching flow method (sodium hypochlorite is added only to secondary amines) and the non-switching flow method (sodium hypochlorite is added to all the amino acids) have been compared. It was concluded that the non-switching flow method ensures high efficiency and reliability. This newly-developed system was applied to the detection of amino acids and gave satisfactory results. |
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Authors:
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Y Ishida; T Fujita; K Asai |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of chromatography Volume: 204 ISSN: 0021-9673 ISO Abbreviation: J. Chromatogr. Publication Date: 1981 Jan |
Date Detail:
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Created Date: 1981-06-13 Completed Date: 1981-06-13 Revised Date: 2000-12-18 |
Medline Journal Info:
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Nlm Unique ID: 0427043 Medline TA: J Chromatogr Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 143-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis* Chromatography, High Pressure Liquid / methods* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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