Document Detail


New crystal structure of the proteasome-dedicated chaperone Rpn14 at 1.6 Å resolution.
MedLine Citation:
PMID:  22691779     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The 26S proteasome is an ATP-dependent protease responsible for selective degradation of polyubiquitylated proteins. Recent studies have suggested that proteasome assembly is a highly ordered multi-step process assisted by specific chaperones. Rpn14, an assembly chaperone for ATPase-ring formation, specifically recognizes the ATPase subunit Rpt6. The structure of Rpn14 at 2.0 Å resolution in space group P6(4) has previously been reported, but the detailed mechanism of Rpn14 function remains unclear. Here, a new crystal structure of Rpn14 with an E384A mutation is presented in space group P2(1) at 1.6 Å resolution. This high-resolution structure provides a framework for understanding proteasome assembly.
Authors:
Sangwoo Kim; Akira Nishide; Yasushi Saeki; Kenji Takagi; Keiji Tanaka; Koichi Kato; Tsunehiro Mizushima
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-04-20
Journal Detail:
Title:  Acta crystallographica. Section F, Structural biology and crystallization communications     Volume:  68     ISSN:  1744-3091     ISO Abbreviation:  Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-06-13     Completed Date:  2012-08-24     Revised Date:  2014-05-11    
Medline Journal Info:
Nlm Unique ID:  101226117     Medline TA:  Acta Crystallogr Sect F Struct Biol Cryst Commun     Country:  England    
Other Details:
Languages:  eng     Pagination:  517-21     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Carrier Proteins / chemistry*,  genetics
Hydrogen Bonding
Models, Molecular
Molecular Sequence Data
Mutation
Protein Structure, Quaternary
Protein Structure, Tertiary
Saccharomyces cerevisiae / chemistry*
Saccharomyces cerevisiae Proteins / chemistry*,  genetics
Structural Homology, Protein
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Rpn14 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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