Document Detail

New cell lines from Lymantria xylina (Lepidoptera: Lymantriidae): characterization and susceptibility to baculoviruses.
MedLine Citation:
PMID:  16989856     Owner:  NLM     Status:  MEDLINE    
Four new cell lines, designated as NTU-LY-1 to -4, respectively, were established from the pupal tissues of Lymantria xylina Swinhoe (Lepidoptera: Lymantriidae). These cell lines have been cultured approximately 80 passages during 2 years in TNM-FH medium supplemented with 8% fetal bovine serum, at a constant temperature of 28 degrees C. Each line consists of three major morphological types: round cells, spindle-shaped cells, and giant cells. The characterization of these cell lines showed that they are different from previously established lines derived from related Lepidopteran species. All new lines were susceptible to the L. xylina multiple nucleopolyhedrovirus (LyxyMNPV) and appeared to have a good potential for studying this virus.
Chih-Yu Wu; Chung-Hsiung Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-09-20
Journal Detail:
Title:  Journal of invertebrate pathology     Volume:  93     ISSN:  0022-2011     ISO Abbreviation:  J. Invertebr. Pathol.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-10-30     Completed Date:  2006-12-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0014067     Medline TA:  J Invertebr Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  186-91     Citation Subset:  IM    
Department of Entomology, National Taiwan University, Taipei, Taiwan, ROC.
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MeSH Terms
Cell Line / cytology*,  virology*
Cell Proliferation
Moths / classification,  cytology*,  virology*
Nucleopolyhedrovirus / growth & development,  pathogenicity*
Pupa / cytology,  growth & development
Virus Replication

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