| New azo dyes as colored isoelectric point markers for isoelectric focusing in acidic pH region. | |
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MedLine Citation:
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PMID: 15624139 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Newly prepared azo compounds and several commercially available indicators were investigated for their applicability as colored isoelectric point (pI) markers for isoelectric focusing (IEF) in the acidic range below pH 5. The majority of compounds described here can serve as primary standards since their pI values were determined by UV-VIS spectrophotometry independently IEF and direct measurement with a pH electrode. Subjected to gel IEF they show narrow and well-observable zones of different colors. Finally, our work resulted in suggestion of a color ladder composed of pI markers covering the pH range from 1.5 to 4.7. |
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Authors:
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Miroslava Stastná; Martin Trávnícek; Karel Slais |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Electrophoresis Volume: 26 ISSN: 0173-0835 ISO Abbreviation: Electrophoresis Publication Date: 2005 Jan |
Date Detail:
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Created Date: 2005-01-10 Completed Date: 2005-06-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8204476 Medline TA: Electrophoresis Country: Germany |
Other Details:
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Languages: eng Pagination: 53-9 Citation Subset: IM |
Affiliation:
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Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech Republic. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Azo Compounds
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chemical synthesis,
chemistry* Coloring Agents / chemical synthesis, chemistry* Hydrogen-Ion Concentration Isoelectric Focusing* Isoelectric Point |
| Chemical | |
Reg. No./Substance:
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0/Azo Compounds; 0/Coloring Agents |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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