Document Detail


New autoantigens in rheumatoid arthritis (RA): screening 8268 protein arrays with sera from patients with RA.
MedLine Citation:
PMID:  18957483     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To identify new IgG autoantibodies in sera from patients with rheumatoid arthritis (RA).
METHODS: We tested serum samples from 19 patients with RA with given human leukocyte antigen (HLA)-DR genotypes, from 7 patients with spondylarthropathy, 2 patients with lupus, 4 patients with systemic sclerosis and 10 healthy individuals on 8268 human protein arrays.
RESULTS: We identified four antigens (peptidyl arginine deiminase 4 (PAD4), protein kinase Cbeta1 (PKCbeta1), phosphatylinositol 4 phosphate 5 kinase type II gamma (PIP4K2C) and v raf murine sarcoma viral oncogene homologue B1 catalytic domain (BRAF)) that were recognised almost uniquely by sera from patients with RA on protein arrays. Using purified proteins, we confirmed that PAD4 and BRAF are recognised almost uniquely by patients with RA.
CONCLUSION: We identified PAD4 and BRAF as RA specific autoantigens.
Authors:
I Auger; N Balandraud; J Rak; N Lambert; M Martin; J Roudier
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-10-28
Journal Detail:
Title:  Annals of the rheumatic diseases     Volume:  68     ISSN:  1468-2060     ISO Abbreviation:  Ann. Rheum. Dis.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-03-16     Completed Date:  2009-04-27     Revised Date:  2012-06-21    
Medline Journal Info:
Nlm Unique ID:  0372355     Medline TA:  Ann Rheum Dis     Country:  England    
Other Details:
Languages:  eng     Pagination:  591-4     Citation Subset:  IM    
Affiliation:
INSERM UMR 639, Parc Scientifique de Luminy, APHM La Conception, Marseille, France. isabelle.auger@medecine.univ-mrs.fr
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MeSH Terms
Descriptor/Qualifier:
Arthritis, Rheumatoid / immunology*
Autoantibodies / blood*,  immunology
Autoantigens / immunology*
Blotting, Western / methods
Case-Control Studies
Enzyme-Linked Immunosorbent Assay / methods
Humans
Hydrolases / blood,  immunology
Immunoglobulin G / immunology*
Protein Array Analysis*
Protein Kinase C / blood,  immunology
Proto-Oncogene Proteins B-raf / blood,  immunology
Chemical
Reg. No./Substance:
0/Autoantibodies; 0/Autoantigens; 0/Immunoglobulin G; EC 2.7.1.-/protein kinase C beta; EC 2.7.11.1/BRAF protein, human; EC 2.7.11.1/Proto-Oncogene Proteins B-raf; EC 2.7.11.13/Protein Kinase C; EC 3.-/Hydrolases; EC 3.5.3.15/peptidylarginine deiminase type IV

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