Document Detail


New assays for monitoring residual HIV burden in effectively treated individuals.
MedLine Citation:
PMID:  23314907     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE OF REVIEW: Measurements of HIV burden have relied upon quantification of viral nucleic acids by real-time PCR (qPCR). To develop and test strategies for eradication, new methods are needed to better characterize residual cellular reservoirs in patients on suppressive antiretroviral therapy (ART). This review summarizes recent advances that may lead to clinically useful tests with improved sensitivity, reproducibility and throughput.
RECENT FINDINGS: HIV DNA remains the most sensitive measure of residual infection, but its low levels are difficult to differentiate from assay noise by qPCR. Digital PCR has begun to improve the precision of existing real-time assays, but there remains a need to distinguish replication-competent proviruses. Rapid technological progress in single-cell analysis is beginning to offer new approaches, notably CyTOF and microengraving, which could provide vastly more information about the composition of the latent reservoir.
SUMMARY: To investigate and assess therapies directed towards eradication, improved assays that simultaneously offer high sensitivity, precision and information content will be needed.
Authors:
Matthew C Strain; Douglas D Richman
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.; Review    
Journal Detail:
Title:  Current opinion in HIV and AIDS     Volume:  8     ISSN:  1746-6318     ISO Abbreviation:  Curr Opin HIV AIDS     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-05     Completed Date:  2013-07-22     Revised Date:  2013-08-30    
Medline Journal Info:
Nlm Unique ID:  101264945     Medline TA:  Curr Opin HIV AIDS     Country:  United States    
Other Details:
Languages:  eng     Pagination:  106-10     Citation Subset:  IM; X    
Affiliation:
University of California San Diego, La Jolla, California 92093-0679, USA.
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MeSH Terms
Descriptor/Qualifier:
Anti-HIV Agents / therapeutic use*
DNA, Viral / analysis,  isolation & purification
Drug Monitoring / methods*
HIV / isolation & purification*
HIV Infections / drug therapy*,  virology*
Humans
Proviruses / isolation & purification
Viral Load / methods*
Grant Support
ID/Acronym/Agency:
AI047745/AI/NIAID NIH HHS; AI080193/AI/NIAID NIH HHS; AI096113/AI/NIAID NIH HHS; AI306214/AI/NIAID NIH HHS; AI69432/AI/NIAID NIH HHS; AI74621/AI/NIAID NIH HHS; P01 AI074621/AI/NIAID NIH HHS; P01 AI080193/AI/NIAID NIH HHS; P30 AI036214/AI/NIAID NIH HHS; R01 AI047745/AI/NIAID NIH HHS; R21 AI047745/AI/NIAID NIH HHS; U01 AI069432/AI/NIAID NIH HHS; U19 AI096113/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Anti-HIV Agents; 0/DNA, Viral
Comments/Corrections

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