Document Detail

New World arenavirus clade C, but not clade A and B viruses, utilizes alpha-dystroglycan as its major receptor.
MedLine Citation:
PMID:  11967329     Owner:  NLM     Status:  MEDLINE    
Alpha-dystroglycan (alpha-DG) has been identified as a major receptor for lymphocytic choriomeningitis virus (LCMV) and Lassa virus, two Old World arenaviruses. The situation with New World arenaviruses is less clear: previous studies demonstrated that Oliveros virus also exhibited high-affinity binding to alpha-DG but that Guanarito virus did not. To extend these initial studies, several additional Old and New World arenaviruses were screened for entry into mouse embryonic stem cells possessing or lacking alpha-DG. In addition, representative viruses were further analyzed for direct binding to alpha-DG by means of a virus overlay protein blot assay technique. These studies indicate that Old World arenaviruses use alpha-DG as a major receptor, whereas, of the New World arenaviruses, only clade C viruses (i.e., Oliveros and Latino viruses) use alpha-DG as a major receptor. New World clade A and B arenaviruses, which include the highly pathogenic Machupo, Guanarito, Junin, and Sabia viruses, appear to use a different receptor or coreceptor for binding. Previous studies with LCMV have suggested the need for a small aliphatic amino acid at LCMV GP1 glycoprotein amino acid position 260 to allow high-affinity binding to alpha-DG. As reported herein, this requirement appears to be broadly applicable to the arenaviruses as determined by more extensive analysis of alpha-DG receptor usage and GP1 sequences of Old and New World arenaviruses. In addition, GP1 amino acid position 259 also appears to be important, since all arenaviruses showing high-affinity alpha-DG binding possess a bulky aromatic amino acid (tyrosine or phenylalanine) at this position.
Christina F Spiropoulou; Stefan Kunz; Pierre E Rollin; Kevin P Campbell; Michael B A Oldstone
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of virology     Volume:  76     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  2002 May 
Date Detail:
Created Date:  2002-04-22     Completed Date:  2002-06-07     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5140-6     Citation Subset:  IM    
Special Pathogens Branch, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
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MeSH Terms
Amino Acid Sequence
Arenaviruses, New World / classification,  genetics,  physiology*
Arenaviruses, Old World / classification,  physiology
Cell Line
Cytoskeletal Proteins / metabolism,  physiology*
Dystrophin / metabolism,  physiology*
Glycoproteins / genetics
Membrane Glycoproteins / metabolism,  physiology*
Molecular Sequence Data
Receptors, Virus / metabolism
Sequence Alignment
Stem Cells / metabolism,  virology*
Viral Envelope Proteins / genetics
Virus Replication
Grant Support
Reg. No./Substance:
0/Cytoskeletal Proteins; 0/Dystrophin; 0/Glycoproteins; 0/Membrane Glycoproteins; 0/Receptors, Virus; 0/Viral Envelope Proteins; 146888-27-9/Dystroglycans

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