Document Detail

A New Technique for Modeling of Hematogenous Osteomyelitis in Pigs: Inoculation into Femoral Artery.
MedLine Citation:
PMID:  23273181     Owner:  NLM     Status:  Publisher    
ABSTRACT A new inoculation technique has been developed and applied in a porcine model of juvenile hematogenous osteomyelitis. Following the success of the model, we describe the inoculation technique in detail to enable its replication in future studies. The technique was based on an anatomical feature of the femoral artery that enables inoculation into the artery using a simple surgical procedure. Inoculation in the femoral artery is advantageous because the localization of lesions constitutes a discriminative model of the naturally occurring hematogenous osteomyelitis in long bones, usually involving femur and tibia in children. The procedure was performed under general anesthesia and consisted of five major steps: (1) Exposure of the right femoral artery, (2) retrograde catheterization, (3) inoculation of bacteria, (4) hemostasis of the arterial puncture site using compression, and (5) suturing of the wound in two layers.
Louise K Johansen; Eiliv L Svalastoga; Dorte Frees; Bent Aalbæk; Janne Koch; Tine M Iburg; Ole L Nielsen; Páll S Leifsson; Henrik E Jensen
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-28
Journal Detail:
Title:  Journal of investigative surgery : the official journal of the Academy of Surgical Research     Volume:  -     ISSN:  1521-0553     ISO Abbreviation:  J Invest Surg     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8809255     Medline TA:  J Invest Surg     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Veterinary Disease Biology, Ridebanevej 3 , Frederiksberg , Denmark.
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