Document Detail


New stepwise cooling system for short-term porcine islet preservation.
MedLine Citation:
PMID:  20467350     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: Porcine islets are the most suitable for xeno-islet transplantation. However, it is necessary to establish an effective preservation method against its fragility. Recently, we developed a new cooling and preservation (Keep and Fresh [KFC]; FUJIYA Co, Tokushima, Japan) system, which can maintain viability of hepatocyte. In this study, we examined the KFC for porcine islet preservation.
METHODS: Isolated porcine islets were preserved in CMRL 1066 culture media with bovine serum at 37°C, 22°C, and 4°C and KFC for 24, 48, and 72 hours. Islet recovery rate, purity, and viability were evaluated.
RESULTS: After 24-hour preservation, the recovery rate was the highest in the KFC, but no significant difference was found. After 48-hour preservation, the recovery rate by the KFC was 73.9% ± 17.3%, which was significantly higher than the other groups (48.7% ± 28.6% at 37°C, P < 0.01; 46.6% ± 15.5% at 22°C, P < 0.01; 61.5% ± 20.0% at 4°C, P < 0.05). After 72-hour preservation, the difference of recovery rate was clearer. In the KFC group, purities and viabilities were the highest among the groups after 24-, 48-, and 72-hour preservation.
CONCLUSIONS: The KFC system significantly improved porcine islet preservation; therefore, the KFC might be useful for porcine islet preservation.
Authors:
Tetsuya Ikemoto; Hirofumi Noguchi; Yasutaka Fujita; Morihito Takita; Masayuki Shimoda; Koji Sugimoto; Andrew Jackson; Bashoo Naziruddin; Mitsuo Shimada; Marlon F Levy; Shinichi Matsumoto
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Pancreas     Volume:  39     ISSN:  1536-4828     ISO Abbreviation:  Pancreas     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-23     Completed Date:  2011-01-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8608542     Medline TA:  Pancreas     Country:  United States    
Other Details:
Languages:  eng     Pagination:  960-3     Citation Subset:  IM    
Affiliation:
Baylor Institute for Immunology Research, Baylor Research Institute, Dallas, TX, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Survival
Islets of Langerhans / physiology*
Islets of Langerhans Transplantation*
Organ Preservation / methods*
Swine

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