| New stepwise cooling system for short-term porcine islet preservation. | |
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MedLine Citation:
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PMID: 20467350 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: Porcine islets are the most suitable for xeno-islet transplantation. However, it is necessary to establish an effective preservation method against its fragility. Recently, we developed a new cooling and preservation (Keep and Fresh [KFC]; FUJIYA Co, Tokushima, Japan) system, which can maintain viability of hepatocyte. In this study, we examined the KFC for porcine islet preservation. METHODS: Isolated porcine islets were preserved in CMRL 1066 culture media with bovine serum at 37°C, 22°C, and 4°C and KFC for 24, 48, and 72 hours. Islet recovery rate, purity, and viability were evaluated. RESULTS: After 24-hour preservation, the recovery rate was the highest in the KFC, but no significant difference was found. After 48-hour preservation, the recovery rate by the KFC was 73.9% ± 17.3%, which was significantly higher than the other groups (48.7% ± 28.6% at 37°C, P < 0.01; 46.6% ± 15.5% at 22°C, P < 0.01; 61.5% ± 20.0% at 4°C, P < 0.05). After 72-hour preservation, the difference of recovery rate was clearer. In the KFC group, purities and viabilities were the highest among the groups after 24-, 48-, and 72-hour preservation. CONCLUSIONS: The KFC system significantly improved porcine islet preservation; therefore, the KFC might be useful for porcine islet preservation. |
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Authors:
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Tetsuya Ikemoto; Hirofumi Noguchi; Yasutaka Fujita; Morihito Takita; Masayuki Shimoda; Koji Sugimoto; Andrew Jackson; Bashoo Naziruddin; Mitsuo Shimada; Marlon F Levy; Shinichi Matsumoto |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Pancreas Volume: 39 ISSN: 1536-4828 ISO Abbreviation: Pancreas Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-23 Completed Date: 2011-01-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8608542 Medline TA: Pancreas Country: United States |
Other Details:
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Languages: eng Pagination: 960-3 Citation Subset: IM |
Affiliation:
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Baylor Institute for Immunology Research, Baylor Research Institute, Dallas, TX, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Survival Islets of Langerhans / physiology* Islets of Langerhans Transplantation* Organ Preservation / methods* Swine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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