| New nucleotide analogues with enhanced signal properties. | |
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MedLine Citation:
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PMID: 20047309 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We describe synthesis and testing of a novel type of dye-modified nucleotides which we call macromolecular nucleotides (m-Nucs). Macromolecular nucleotides comprise a nucleotide moiety, a macromolecular linear linker, and a large macromolecular ligand carrying multiple fluorescent dyes. With incorporation of the nucleotide moiety into the growing nucleic acid strand during enzymatic synthesis, the macromolecular ligand together with the coupled dyes is bound to the nucleic acid. By the use of this new class of modified nucleotides, signals from multiple dye molecules can be obtained after a single enzymatic incorporation event. The modified nucleotides are considered especially useful in the fields of nanobiotechnology, where signal stability and intensity is a limiting factor. |
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Authors:
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Dmitry Cherkasov; Thorsten Biet; Englbert B?uml; Walther Traut; Michael Lohoff |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Bioconjugate chemistry Volume: 21 ISSN: 1520-4812 ISO Abbreviation: Bioconjug. Chem. Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2010-01-20 Completed Date: 2010-05-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9010319 Medline TA: Bioconjug Chem Country: United States |
Other Details:
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Languages: eng Pagination: 122-9 Citation Subset: IM |
Affiliation:
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Institute for Medical Microbiology, Philipps University of Marburg, Hans-Meerweinstrasse 2, 35043 Marburg, Germany. cherkasovd07@yandex.ru |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Biotechnology / methods* Enzymes / metabolism Fluorescein / chemistry Fluorescent Dyes / analysis*, chemistry* Ligands Nanotechnology / methods* Nucleotides / analysis*, chemistry* Phycoerythrin / chemistry Polyethylene Glycols / chemistry Spectrometry, Fluorescence Streptavidin / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Enzymes; 0/Fluorescent Dyes; 0/Ligands; 0/Nucleotides; 0/Polyethylene Glycols; 11016-17-4/Phycoerythrin; 2321-07-5/Fluorescein; 9013-20-1/Streptavidin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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