| A New Multistep Induction Protocol for the Transdifferentiation of Bone marrow Stromal Stem Cells into GABAergic Neuron-Like Cells. | |
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MedLine Citation:
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PMID: 23279829 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Background: Bone marrow stromal stem cells (BMSC) are appropriate source of multipotent stem cells that are ideally suited for use in various cell-based therapies. It can be differentiated into neuronal-like cells under appropriate conditions. This study examined the effectiveness of co-stimulation of creatine and retinoic acid in increasing the differentiation of BMSC into GABAergic neuron-like cells (GNLC). Methods: BMSC isolated from the femurs and tibias of adult rats were cultured in DMEM/F12 medium supplemented with 10% FBS, pre-induced using β-mercaptoethanol β-ME) and induced using retinoic acid (RA) and creatine. Immunostaining of neurofilament 200 kDa, neurofilament 160 kDa, nestin, fibronectin, Gamma-amino butyric acid (GABA) and glutamic acid decarboxylase (GAD) 65/67 were used to evaluate the transdifferentiation of BMSC into GNLC and to evaluate the effectiveness of pre-induction and induction assays. The expression of genes that encode fibronectin, octamer-binding transcription factor 4 (Oct-4), GAD 65/67 and the vesicular GABA transporter was examined in BMSC and GNLC by using RT-PCR assays during transdifferentiation of BMSC into GLNC. Results: Co-stimulation with RA and creatine during the induction stage doubled the rates of GABAergic differentiation compared with induction using creatine alone, resulting in a 71.6% yield for GLNC. RT-PCR showed no expression of Oct-4 and fibronectin after the induction stage. Conclusion: The results of this study showed that the application of β-ME, RA, and creatine induced the transdifferentiation of BMSC into GLNC. |
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Authors:
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Shahram Darabi; Taki Tiraihi; Alireza Delshad; Majid Sadeghizadeh |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Iranian biomedical journal Volume: 17 ISSN: 2008-823X ISO Abbreviation: Iran. Biomed. J. Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-01-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9814853 Medline TA: Iran Biomed J Country: Iran |
Other Details:
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Languages: eng Pagination: 8-14 Citation Subset: IM |
Affiliation:
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Dept. of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. takialtr@modares.ac.ir. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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