Document Detail

A new multistep induction protocol for the transdifferentiation of bone marrow stromal stem cells into GABAergic neuron-like cells.
MedLine Citation:
PMID:  23279829     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Bone marrow stromal stem cells (BMSC) are appropriate source of multipotent stem cells that are ideally suited for use in various cell-based therapies. It can be differentiated into neuronal-like cells under appropriate conditions. This study examined the effectiveness of co-stimulation of creatine and retinoic acid in increasing the differentiation of BMSC into GABAergic neuron-like cells (GNLC).
METHODS: BMSC isolated from the femurs and tibias of adult rats were cultured in DMEM/F12 medium supplemented with 10% FBS, pre-induced using β-mercaptoethanol β-ME) and induced using retinoic acid (RA) and creatine. Immunostaining of neurofilament 200 kDa, neurofilament 160 kDa, nestin, fibronectin, Gamma-amino butyric acid (GABA) and glutamic acid decarboxylase (GAD) 65/67 were used to evaluate the transdifferentiation of BMSC into GNLC and to evaluate the effectiveness of pre-induction and induction assays. The expression of genes that encode fibronectin, octamer-binding transcription factor 4 (Oct-4), GAD 65/67 and the vesicular GABA transporter was examined in BMSC and GNLC by using RT-PCR assays during transdifferentiation of BMSC into GLNC.
RESULTS: Co-stimulation with RA and creatine during the induction stage doubled the rates of GABAergic differentiation compared with induction using creatine alone, resulting in a 71.6% yield for GLNC. RT-PCR showed no expression of Oct-4 and fibronectin after the induction stage.
CONCLUSION: The results of this study showed that the application of β-ME, RA, and creatine induced the transdifferentiation of BMSC into GLNC.
Shahram Darabi; Taki Tiraihi; AliReza Delshad; Majid Sadeghizadeh
Related Documents :
23617469 - Cd43 expression is associated with inferior survival in the non-germinal centre b-cell ...
23792279 - Phenotypic characterization and in vitro propagation and transplantation of the nile ti...
23291919 - Paroxetine up-regulates neurogenesis in hippocampus-derived neural stem cell from fetal...
23659669 - Rapid interferon-gamma release from natural killer cells induced by a streptococcal com...
24356089 - Toll-like receptor 4 is required for the cytotoxicity of cytokine-induced killer cells.
23840769 - Dose-dependent induction of murine th1/th2 responses to sheep red blood cells occurs in...
3264529 - Regulatory effects of human ige-binding factors in the ige synthesis by human and rat l...
24497109 - Better safe than sorry: tob1 employs multiple parallel regulatory pathways to keep th17...
12974779 - The role of dendritic cells in the pathogenesis of hiv-1 infection.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Iranian biomedical journal     Volume:  17     ISSN:  2008-823X     ISO Abbreviation:  Iran. Biomed. J.     Publication Date:  2013  
Date Detail:
Created Date:  2013-01-02     Completed Date:  2013-06-25     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  9814853     Medline TA:  Iran Biomed J     Country:  Iran    
Other Details:
Languages:  eng     Pagination:  8-14     Citation Subset:  IM    
Dept. of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Bone Marrow Cells / cytology
Cell Transdifferentiation / drug effects*
Cells, Cultured
Fibronectins / biosynthesis
GABAergic Neurons / cytology,  metabolism*
Glutamate Decarboxylase / biosynthesis
Mesenchymal Stromal Cells / cytology,  metabolism*
Octamer Transcription Factor-3 / biosynthesis
Rats, Sprague-Dawley
Vesicular Inhibitory Amino Acid Transport Proteins / biosynthesis
Reg. No./Substance:
0/Fibronectins; 0/Octamer Transcription Factor-3; 0/Vesicular Inhibitory Amino Acid Transport Proteins; 0/vesicular GABA transporter; 302-79-4/Tretinoin; 57-00-1/Creatine; 60-24-2/Mercaptoethanol; EC Decarboxylase; EC decarboxylase 2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Cloning, expression and characterization of recombinant exotoxin A-flagellin fusion protein as a new...
Next Document:  Improved real-time rt-PCR assays of two colorectal cancer peripheral blood mRNA biomarkers: a pilot ...