Document Detail


A new model for portal protein profile analysis in course of ileal intraluminal bile acid infusion using an in situ perfused rat intestine.
MedLine Citation:
PMID:  21568879     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Due to the importance of intestinal transport in pharmacological studies and the emerging role of intestinal signaling activity in the gut-liver axis, we have developed a new method to investigate intestinal transport and liver signaling using cell and serum free mesenteric perfusion system in the rat. The method regarding bile acid active absorption was validated, then, the portal venous content was examined for fibroblast growth factor 15(FGF15), a putative signaling protein produced by the ileal enterocytes following bile acid absorption. After isolation and cannulation of the relevant vessels (abdominal aorta and portal vein), the abdominal aorta and the terminal ileum were infused with respectively Krebs-Ringer solution and tauroursodeoxycholate (TUDCA) and the absorption was assessed by its recovery in the portal vein. After immunoblot, liquid chromatography and mass spectrometry analysis were performed both on gel bands digestion products and on portal outflow samples in order to evaluate if negligible amounts of FGF15 were present in the portal circulation. TUDCA absorption was efficient, intestinal morphology and oxygen consumption were normal. Despite accurate analysis, we could not find FGF15. Our method proved to be reliable for studying the active bile acid absorption. It is also suitable to identify molecules produced by enterocytes and transferred to the portal circulation in response to absorption of different substances such as nutrients or drugs. Since FGF15 was not recovered we suggest the possibilities that this protein is produced in very little amounts, poorly transferred outside the cell, or that it is extremely unstable and rapidly degraded.
Authors:
Marco Montagnani; Matvey Tsivian; Flavia Neri; Ido Ben Zvi; Irina Mantovani; Paolo Nanni; Marco Benevento; Patrizia Simoni; Antonella Marangoni; Milena Pariali; Romana Fato; Christian Bergamini; Serena Leoni; Francesco Azzaroli; Giuseppe Mazzella; Bruno Nardo; Enrico Roda; Rita Aldini
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Medicinal chemistry (Shāriqah (United Arab Emirates))     Volume:  7     ISSN:  1875-6638     ISO Abbreviation:  Med Chem     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-08-02     Completed Date:  2011-12-20     Revised Date:  2012-03-14    
Medline Journal Info:
Nlm Unique ID:  101240303     Medline TA:  Med Chem     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  257-64     Citation Subset:  IM    
Affiliation:
Dipartimento di Medicina Clinica, Centro di Ricerca Biomedica Applicata-Policlinico S.Orsola, Università di Bologna, Bologna, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bile / metabolism
Cell Respiration / drug effects
Cholagogues and Choleretics / administration & dosage,  metabolism
Enterocytes / drug effects,  metabolism
Fibroblast Growth Factors / blood*
Ileum / drug effects,  metabolism
Intestinal Absorption*
Intestines / drug effects,  metabolism*
Isotonic Solutions
Liver / metabolism
Male
Models, Animal
Portal Vein
Rats
Rats, Sprague-Dawley
Taurochenodeoxycholic Acid / administration & dosage,  metabolism*
Chemical
Reg. No./Substance:
0/Cholagogues and Choleretics; 0/Isotonic Solutions; 0/Krebs-Ringer solution; 14605-22-2/tauroursodeoxycholic acid; 516-35-8/Taurochenodeoxycholic Acid; 62031-54-3/Fibroblast Growth Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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