Document Detail


A new insight into application for barley chromosome addition lines of common wheat: achievement of stigmasterol accumulation.
MedLine Citation:
PMID:  21951468     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Barley (Hordeum vulgare) has a much higher content of bioactive substances than wheat (Triticum aestivum). In order to investigate additive and/or synergistic effect(s) on the phytosterol content of barley chromosomes, we used a series of barley chromosome addition lines of common wheat that were produced by normal crossing. In determining the plant sterol levels in 2-week-old seedlings and dry seeds, we found that the level of stigmasterol in the barley chromosome 3 addition (3H) line in the seedlings was 1.5-fold higher than that in the original wheat line and in the other barley chromosome addition lines, but not in the seeds. Simultaneously, we determined the overall expression pattern of genes related to plant sterol biosynthesis in the seedlings of wheat and each addition line to assess the relative expression of each gene in the sterol pathway. Since we elucidated the CYP710A8 (cytochrome P450 subfamily)-encoding sterol C-22 desaturase as a key characteristic for the higher level of stigmasterol, full-length cDNAs of wheat and barley CYP710A8 genes were isolated. These CYP710A8 genes were mapped on chromosome 3 in barley (3H) and wheat (3A, 3B, and 3D), and the expression of CYP710A8 genes increased in the 3H addition line, indicating that it is responsible for stigmasterol accumulation. Overexpression of the CYP710A8 genes in Arabidopsis increased the stigmasterol content but did not alter the total sterol level. Our results provide new insight into the accumulation of bioactive compounds in common wheat and a new approach for assessing plant metabolism profiles.
Authors:
Jianwei Tang; Kiyoshi Ohyama; Kanako Kawaura; Hiromi Hashinokuchi; Yoko Kamiya; Masashi Suzuki; Toshiya Muranaka; Yasunari Ogihara
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-09-27
Journal Detail:
Title:  Plant physiology     Volume:  157     ISSN:  1532-2548     ISO Abbreviation:  Plant Physiol.     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-04     Completed Date:  2012-02-27     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1555-67     Citation Subset:  IM    
Affiliation:
Kihara Institute for Biological Research, Yokohama City University, Yokohama 244-0813, Japan.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Arabidopsis / genetics
Biosynthetic Pathways
Chromosomes, Plant / metabolism*
Crosses, Genetic*
Gene Expression Regulation, Plant
Genes, Plant / genetics
Genetic Techniques*
Hordeum / genetics*,  metabolism
Molecular Sequence Data
Plant Leaves / metabolism
Plant Proteins / chemistry,  genetics,  metabolism
Plants, Genetically Modified
Seedling / genetics,  metabolism
Seeds / metabolism
Sitosterols / metabolism
Stigmasterol / metabolism*
Triticum / genetics*,  metabolism
Chemical
Reg. No./Substance:
0/Plant Proteins; 0/Sitosterols; 5LI01C78DD/gamma-sitosterol; 83-48-7/Stigmasterol
Comments/Corrections

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