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A New Generation Microarray for the Simultaneous Detection and Identification of Yersinia pestis and Bacillus anthracis in Food.
MedLine Citation:
PMID:  23125935     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
The use of microarrays as a multiple analytic system has generated increased interest and provided a powerful analytical tool for the simultaneous detection of pathogens in a single experiment. A wide array of applications for this technology has been reported. A low density oligonucleotide microarray was generated from the genetic sequences of Y. pestis and B. anthracis and used to fabricate a microarray chip. The new generation chip, consisting of 2,240 spots in 4 quadrants with the capability of stripping/rehybridization, was designated as "Y-PESTIS/B-ANTHRACIS 4x2K Array." The chip was tested for specificity using DNA from a panel of bacteria that may be potentially present in food. In all, 37 unique Y. pestis-specific and 83 B. anthracis-specific probes were identified. The microarray assay distinguished Y. pestis and B. anthracis from the other bacterial species tested and correctly identified the Y. pestis-specific oligonucleotide probes using DNA extracted from experimentally inoculated milk samples. Using a whole genome amplification method, the assay was able to detect as low as 1 ng genomic DNA as the start sample. The results suggest that oligonucleotide microarray can specifically detect and identify Y. pestis and B. anthracis and may be a potentially useful diagnostic tool for detecting and confirming the organisms in food during a bioterrorism event.
Authors:
Noriko Goji; Trevor Macmillan; Kingsley Kwaku Amoako
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Publication Detail:
Type:  Journal Article     Date:  2012-10-18
Journal Detail:
Title:  Journal of pathogens     Volume:  2012     ISSN:  2090-3065     ISO Abbreviation:  J Pathog     Publication Date:  2012  
Date Detail:
Created Date:  2012-11-05     Completed Date:  2012-11-06     Revised Date:  2013-03-11    
Medline Journal Info:
Nlm Unique ID:  101571433     Medline TA:  J Pathog     Country:  Egypt    
Other Details:
Languages:  eng     Pagination:  627036     Citation Subset:  -    
Affiliation:
Canadian Food Inspection Agency, National Centres for Animal Disease, Lethbridge Laboratory, P.O. Box 640, Lethbridge, AB, Canada T1J 3Z4.
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