| A New Generation Microarray for the Simultaneous Detection and Identification of Yersinia pestis and Bacillus anthracis in Food. | |
| | |
MedLine Citation:
|
PMID: 23125935 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
|
The use of microarrays as a multiple analytic system has generated increased interest and provided a powerful analytical tool for the simultaneous detection of pathogens in a single experiment. A wide array of applications for this technology has been reported. A low density oligonucleotide microarray was generated from the genetic sequences of Y. pestis and B. anthracis and used to fabricate a microarray chip. The new generation chip, consisting of 2,240 spots in 4 quadrants with the capability of stripping/rehybridization, was designated as "Y-PESTIS/B-ANTHRACIS 4x2K Array." The chip was tested for specificity using DNA from a panel of bacteria that may be potentially present in food. In all, 37 unique Y. pestis-specific and 83 B. anthracis-specific probes were identified. The microarray assay distinguished Y. pestis and B. anthracis from the other bacterial species tested and correctly identified the Y. pestis-specific oligonucleotide probes using DNA extracted from experimentally inoculated milk samples. Using a whole genome amplification method, the assay was able to detect as low as 1 ng genomic DNA as the start sample. The results suggest that oligonucleotide microarray can specifically detect and identify Y. pestis and B. anthracis and may be a potentially useful diagnostic tool for detecting and confirming the organisms in food during a bioterrorism event. |
| | |
Authors:
|
Noriko Goji; Trevor Macmillan; Kingsley Kwaku Amoako |
Related Documents
:
|
15254125 - Runoff phosphorus losses as related to phosphorus source, application method, and appli... 7752105 - Concentration factors for cs-137 in marine algae from japanese coastal waters. 23603775 - An urban metabolism and ecological footprint assessment of metro vancouver. 11429775 - Phytoplankton group dynamics in the bay of marseilles during a 2-year survey based on a... 16028975 - Preclinical evaluation of rapeseed, raspberry, and pine bark phenolics for health relat... 6517565 - Acculturation to canadian foods by chinese immigrant boys: changes in the perceived fla... |
Publication Detail:
|
Type: Journal Article Date: 2012-10-18 |
Journal Detail:
|
Title: Journal of pathogens Volume: 2012 ISSN: 2090-3065 ISO Abbreviation: J Pathog Publication Date: 2012 |
Date Detail:
|
Created Date: 2012-11-05 Completed Date: 2012-11-06 Revised Date: 2013-03-11 |
Medline Journal Info:
|
Nlm Unique ID: 101571433 Medline TA: J Pathog Country: Egypt |
Other Details:
|
Languages: eng Pagination: 627036 Citation Subset: - |
Affiliation:
|
Canadian Food Inspection Agency, National Centres for Animal Disease, Lethbridge Laboratory, P.O. Box 640, Lethbridge, AB, Canada T1J 3Z4. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Diagnostic role of chromosomal instability in melanoma.
Next Document: Periarticular injection with bupivacaine for postoperative pain control in total knee replacement: a...