Document Detail


New features of invasive candidiasis in humans: amyloid formation by fungi and deposition of serum amyloid P component by the host.
MedLine Citation:
PMID:  22802434     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Invasive candidiasis occurs in the gastrointestinal tract, especially in neutropenic patients. We were interested in determining whether invasive fungi formed amyloid in humans as they are known to do in vitro. We also sought to characterize the consequence(s) of such amyloid formation.
METHODS: Tissue from 25 autopsy patients with invasive candidiasis of the gastrointestinal tract was stained with amyloidophilic dyes and for the presence of serum amyloid P component (SAP). Confirmation of the interaction of SAP and Candida was demonstrated using Candida albicans and mutants for amyloid formation.
RESULTS: Amyloid was present on the cellular surface of fungi invading gut tissue. Moreover, SAP bound to the fungal cell walls, confirming the presence of amyloid. In vitro observations showed SAP bound avidly to fungi when amyloid formed in fungal cell walls. An unexpected result was the lack of host neutrophils in response to the invading fungi, not only in neutropenic patients but also in patients with normal or increased white blood counts.
CONCLUSIONS: We report the first demonstration of functional fungal amyloid in human tissue and the binding of SAP to invading fungi. It is postulated that fungal amyloid, SAP, or a complex of the proteins may inhibit the neutrophil response.
Authors:
Kevin B Gilchrist; Melissa C Garcia; Richard Sobonya; Peter N Lipke; Stephen A Klotz
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Publication Detail:
Type:  Journal Article     Date:  2012-07-16
Journal Detail:
Title:  The Journal of infectious diseases     Volume:  206     ISSN:  1537-6613     ISO Abbreviation:  J. Infect. Dis.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-10     Completed Date:  2013-01-04     Revised Date:  2013-11-06    
Medline Journal Info:
Nlm Unique ID:  0413675     Medline TA:  J Infect Dis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1473-8     Citation Subset:  AIM; IM    
Affiliation:
Department of Pathology, University of Arizona, Tucson, AZ, USA.
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MeSH Terms
Descriptor/Qualifier:
Candida albicans / chemistry*,  metabolism
Candidiasis, Invasive / microbiology,  pathology*
Gastrointestinal Diseases / microbiology,  pathology*
Histocytochemistry
Humans
Microscopy
Plaque, Amyloid / pathology*
Serum Amyloid P-Component / analysis*
Staining and Labeling
Grant Support
ID/Acronym/Agency:
R01 GM098616/GM/NIGMS NIH HHS; SC1 GM083756/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Serum Amyloid P-Component
Comments/Corrections
Comment In:
J Infect Dis. 2012 Nov;206(9):1339-41   [PMID:  22927458 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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